Effects of bacteria and nitrogen fertilizer on promoting maize (Zea mays L.) growth, yield and chemical constituents

Hind Ahmed M. Abd El Magid, Abdelsalam Kamil Abdelsalam, Nahid Abd Alfatah Mohamed Khalil, Ahmed M.E. Rugheim, Mohammed Mahgoub Hassan


A field experiment was carried out during two successive summer seasons (2016/ 2017- 2017/2018), at the College of Agricultural Studies, Sudan University of Sciences and Technology, to assess the effect of bacterial strains, nitrogen and their combinations on the performances of two maize genotypes Hudibi2 (C1) and ZML309 (C2). The form of two types of bacterial strains Bacillus megatherium var. phosphorous + Azotobacter spp + Azospirillium spp (B1) and Bacillus megatherium var. phosphorous + Azotobacter spp + Flavobacterium spp (B2), nitrogen applied at rates of 80kg/f (N) plus control. The treatments were arranged in a Randomized Complete Block Design (RCBD) in four replicates. The results showed that the combinations of B1 and B2 with N increased plant growth compared to B1, B2, and N each alone at both seasons. The general trend was the combinations (B2+N, B1+N) had significant effect on all growth parameters in both seasons. Yield components increased with combination (B2+N) followed by (B1+N), the maximum seed yield (5.0 and 2.8t/ha) was achieved by the interaction of (C2*B2+N) in the first season and (C2*B1+N) in the second season, respectively. Generally, in among all treatments, the combination of B2+N increased nitrogen content, crude protein and fiber irrespective to maize genotypes. Moreover, C2 showed the highest values as compared to C1. In conclusion, improvement in maize plant growth and yield are more prominent and significant when genotype ZML309 inoculated with Bacillus megatherium var. phosphorous + Azotobacter spp + Flavobacterium spp and supplemented with 80kg/f nitrogen.


Azotobacter, Azospirillium, Flavobacterium, Maize, Nitrogen%, Protein, Fiber

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Abdalla A.S., Osman A.G., Abdelgani M.E, Rug-heim A.M.E., Effects of biological and mineral fertilization on nodulation, nitrogen and phos-phorus content and yield of chick pea (Cicer arietinum L.), Adv. Environ. Biol., 2011, 5(9): 2886-2894.

A.B.P.S.D., Statistical information of Nepalese Agriculture 2007/2008. Agribusiness Promotion and Statistics Division, Kathmandu, Nepal, 2008, pp 127.

Ahemad M., Kibret M., Mechanisms and appli-cations of plant growth promoting rhizobacteria: Current perspective. J. King Saud Uni. – Sci., 2014, 26, 01-20.

Akbari P., Ghalavand A., Sanavi S.M., Effects of different nutrition systems (Organic, Chemical and Integrated) and biofertilizer on yield and other growth traits of sunflower (Helianthus annus L.), Elec. J. Sust. Agri., 2009, 19, 4-93.

Alvarez R., Grigera S., Analysis of soil fertility and management effects on yields of wheat and corn in the rolling pampa of Argentina, J. Agron. Cropsci., 2005, 191, 321-329.

AOAC, Association of Official Analytical Chem-ist, Official Methods of Analysis, 15th ed. Wash-ington, DC, 1990, pp 648.

Aquino J.P.A., Junior F.B.M., Antunes J.E.L., Fig-ueiredo M.V.B, Neto F.A, Araujo A.S.F., Plant growth-promoting endophytic bacteria on maize and sorghum. Pesq. Agropec. Trop., 2019, 49, 1-9.

Arruda L., Beneduzi A., Martins A., Lisboa B., Lopes C., Bertolo F., Passaglia L.M.P., Vargas L.K. Screening of rhizobacteria isolated from maize (Zea mays L.) in Rio Grande do Sul state (south Brazil) and analysis of their potential to improve plant growth. App. Soil Ecol., 2013, 63(1), 15-22.

Azab M.E., Dewiny C.Y., Effect of bio and mine-ral nitrogen fertilizer with different levels on growth, yield and quality of maize plants. J. Innov. Pharm. Bio. Sci., 2018, 5(2), 70-75.

CGIAR., Maize Agri-Food Systems Proposal 2017 -2022 the Consultative Group for International Agricultural Research, 2016.

Chattha M.B., Iqbal A., Chattha M.U., Hassan M.U., Khan I., Ashraf I., Faisal., Usman M. PGPR inoculated-seed increases the productivity of forage sorghum under fertilized conditions, J. Basic App. Sci., 2017, 13, 150-153.

Crozier A., Arruda P., Jasmim J.M., Monteiro A.M., Sandberg G., Analysis of indole-3-acetic acid and related indoles in culture media from Azospirillum lipoferum and Azospirillum brasili-ense. App. Envi. Microbiol., 1988, 54, 2833-2837.

Dakhly O.F, Abdel Mageed Y.T, Hassan E.A., Effect of nitrogen, organic fertilizers and new Azotobacter trans formants on squash, Minia J. Agric. Res. and Dev., 2004, 24(1), 1-30.

El Toum G.A.M., Effect of Nitrogen and Sowing Methods on Growth, Yield and Yield Efficiency of Three Maize (Zea mays L.) Cultivars under Irrigation. PhD thesis, Sudan University of Science and Technology, Sudan, 2016.

Garg P., Gupta A., Satya S., Vermicomposting of different type of waste using Eiseniafetida: A complementary study. Biores. Tech., 2005, 97, 391-395.

Gholami A., Biyari A., Gholipoor M., Rahmani H. A, Growth promotion of maize (Zea mays L.) by plant-growth promoting rhizobacteria under fie-ld conditions. Comm. Soil Sci. Plant Ana., 2012, 43 (9), 1263-1272.

Goswami S.P., Maurya B.R., Dubey A.N., Influ-ence of potassium solubilizing bacteria (KSB) and sources of potassium on growth and yield of maize. Ann. Agric. Res., 2019, 40 (4), 1-7.

Hellal F.A., Mahfouz S.A., Hassan F.A.S., Partial substitution of mineral nitrogen fertilizer by bio-fertilizer on (Anethum graveolens L.) plant, Agric. Biol. J. N. Am., 2011, 2(4), 652-660.

Helmy A.M., Response of seed irradiation with gamma ray, N-fertilization and bio-fertilization of barley (Hordeum vulgare L.) grown on a sand soil, J. Soil Sci. and Agric. Eng., 2014, 5(1), 1-18.

Javeed H.M.R., Qamar R., Rehman A., Ali M., Rahman A., Farooq M., Zamir S.I., Nadeem M., Cheema M.A., Shehzad M., Zakir A., Aqeel M., Iqbal A., Hussain M., Improvement in Soil Char-acteristics of Sandy Loam Soil and Grain Quality of Spring Maize by Using Phosphorus Solubli-zing Bacteria, Sustain., 2019, 11, 7049.

Lin Y., Watts D.B., Kloepper J.W., Torbert H.A., Influence of plant growth-promoting rhizobacte-ria on corn growth under different fertility, Sour-ces. Comm. Soil Sci. Plant Ana., 2018, 49(10), 1239-1255.

Mahato S., Neupane S., Comparative study of impact of Azotobacter and Trichoderma with other fertilizers on maize growth, J. Maize Res. Dev., 2017, 3(1), 1-16.

Metin T.A., Medine G.B., Ramazan C.C., Taskin O.F, Sahin D., The effect of PGPR strain on wheat yield and quality parameters. Proceeding of Wor-ld Congress of Soil Science, Soil Solution for a Changing World, Brisbane, Australia, 1-6: 2010.

Mohammed S.S., Osman A.G., Mohammed A.M., Abdalla A.S., Sherif A.M., Rugheim A.M.E., Effe-cts of organic and microbial fertilization on whe-at growth and yield, Int. Res. J. Agri. Sci. Soil Sci., 2012, 2(4), 149-154.

Molina-Romero D., Baez A., Quintero-Hernández V., Castañeda-Lucio M., Fuentes Ramírez L.E., Bustillos-Cristales M., Rodríguez-And-rade O., García Y.E.M, Munive A, Muñoz-Rojas J., Comp-atible bacterial mixture, tolerant to desiccation, improves maize plant growth, PLoS one, 2017, 12 (11), 1-8.

Osman A.G., Hassan M.M., Rugheim A.M.E., Abdelgani M.E., Babiker A.E., Effects of organic and microbial fertilizers on Striga hermonthica in maize. Univ. J. Agri. Res., 2013, 1(2), 24-29.

Osman A.G., Rugheim A.M.E., Elsoni E.M., Effe-cts of biofertilization on nodulation, nitrogen and phosphorus content and yield of pigeon pea (Caj-anus cajan L.). Adv. Envi. Biol., 2011, 5 (9), 2742 -2749.

Prajapati K., Impact of potassium solubilizing bacteria on growth and yield of mung bean Vigna radiate. Ind. J. App. Res., 2016, 6(2), 390-392.

Rugheim A.M.E., Abdelgani M.E., Effects of mic-robial and chemical fertilization on yield and seed quality of faba bean (Vicia faba). Int. Food Res. J., 2012, 19 (2), 417-422.

Rugheim A.M.E., Taha K.E.M., Ali S.E.A., Influ-ence of nitrogen fixing and phosphorus solubili-zing bacteria co-inoculation on fenugreek sym-biotic properties, growth and yield. Int.J. Horti., Agri. and Food Sci., 2017, 1(3), 42-47.

Saber R.A., Sharaf A., Biochemical studies on the effect of some biofertilizers on the chemical con-stituents in wheat grains, Int. J. Sci. Res., 2013, 4, 2319-7064.

Shanthi J., Santhi V., Ramya S., Balagurunathan R., Effect of Azotobacter spp. and phosphorbacter bio inoculants on the growth of sunflower (Hel-ianthus annuus L.), World J. Agri. Res. Sci., 2012, 8(2), 218-222.

Sosulski F.W., Imafidon G.I., Amino-acid compo-sition and nitrogen to protein conversion factors for animal plant foods, J. Agri. Food Chem., 1990, 38, 1351-1356.

Tagne A., Feujio T.P., Sonna C., Essential oil and plant extracts as potential substitutes to synthetic fungicides in the control of fungi, International Conference Diversifying Crop Protection, La Gra-nde-Motte, France, 12-15 October 2008.

Ukonze J.A., Akor V.O., Ndubuaku U.M., Comp-arative analysis of three different spacing on the performance and yield of late maize cultivation in Etche local government area of Rivers State, Nigeria, African J. Agri. Res., 2016, 11(13), 1187-1193.

Widawati S., Suliasih M., The effect of plant gro-wth promoting rhizobacteria (PGPR) on germi-nation and seedling growth of Sorghum bicolor L. Moench, Earth and Envi. Sci., 2018, 166, 12-22.

Wu S.C., Cao Z.H., Li Z.G., Cheung KC, Effect of bio-fertilizers containing N fixer P and K solubi-lizers and AM fungi on maize growth: a greenhouse trial, Geoderma. 2005, 125, 155-166.

DOI: https://doi.org/10.5281/aps.2020.9.6.1

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