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In vitro screening for antiacetylcholinesterase and antioxidant activity of Piper longum L.

Navi Ranjan, Preety Sinha, Manorma Kumari


Acetylcholinesterase (AChE) inhibition and antioxidant activity are considered to be highly correlated with Alzheimer’s disease (AD) treatment. The present study was designed to investigate the antioxidant and acetylcholinesterase inhibitory activity of Piper longum L. Properly identified powdered plant material was extracted successively using methanol as a solvent. Acetylcholinesterase inhibitory activity was measured with modified Ellman’s method at 405 nm and antioxidant activity measured based on 1, 1- diphenyl-2-picrylhydrazil (DPPH) free radical scavenging test at 517 nm. Percentage inhibition for AChE ranged from 31.28±0.12 to 62.26±0.05 whereas DPPH radical scavenging percentage ranged from 14.40±0.72 to 37.95±0.67. Results reveal that plant extracts studied possess anti-oxidant properties. Most potent extracts could be a lead to novel antioxidants and acetylcholinesterase inhibitors for the treatment of AD.


Antioxidants, Acetylcholinesterase inhibition, Alzheimer’s disease, DPPH, Piper longum L.

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