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In vitro callus induction in inflorescence segments of medicinally important endangered plant Rauwolfia serpentina (L.) Benth. ex Kurz – a step towards ex situ conservation

Saranjeet Kaur

Abstract


The regenerative competence of inflorescence segments of Rauwolfia serpentina was assessed in M (Mitra et al., 1976) medium and its combinations with growth adjuncts such as cytokinins [N6-benzyladenine (6-BA - 0.5, 1.0 mg l-1), furfurylaminopurine (Kn-0.5, 1.0mg l-1) and auxin [α-naphthalene acetic acid (NAA - 0.5, 1.0 mg l-1)]. Inflorescence segments (1.00 cm in length) were used as explants. The regeneration response was obligatory to the use of growth regulators in the medium. Cytokinins favoured shoot bud mediated pathway of plantlet development and BAP favoured early shooting. The explants callused in NAA enriched medium. The callus was best maintained in NAA (0.5 mg l-1) through repeated sub-culturing. The entire plant of R. serpentina is the target of massive commercial collections resulting into shrinking habitats. Present study is the first ever report on the successful use of inflorescence segments for ex situ conservation of R. serpentina.

Keywords


Auxin, Callus, Cytokinins, Endangered, Medicinal plant

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References


Ahmad S, M N Amin, M A K Azad and M A Mosaddik. “Microprpagation and Plant regeneration of Rauwolfia serpentina by tissue culture technique.” Pakistan Journal of Biological Sciences 5(2002): 75-79.

Anitha S and B D R Kumari. “Stimulation of reserpine biosynthesis in the callus of Rauwolfia tetraphyla L. by precursor feeding.” African Journal of Biotechnology 5(2006): 659-661.

Arora R and S S Bhojwani. “In vitro propagation and low temperature storage of Saussurea lappa C.B. Clarke-an endangered medicinal plant.” Plant Cell Reports 8(1989): 44-47.

Benavides M P and O H Caso. “Plant regeneration and thiophin formation in tissue cultures of Tagetes mendocina.” Plant Cell Tissue and Organ Culture 35(1993): 211-215.

Bhatara V S, J N Sharma, S Gupta and Y K Gupta. “Rauwolfia serpentina: The first antipsychotic.” American Journal of Psychiatry 154(1997): 894-896.

Brisibe E A, A Gajdosova, A Olesen and S B Andersen. “Cytodifferentiation and transformation of embryogenic callus lines derived from anther culture of wheat.” Journal of Experimental Botany 51(2000): 187-196.

CITES (2017) (https://cites.org/eng/app/appendices.php).

Frame B R, P R Drayton, S V Bagnall, C J Lewnau, W P Bullock, H M Wilson, J M Dunwell, J A Thompson and K Wang. “Production of fertile transgenic maize plants by silicon carbide whisker-mediated transformation.” The Plant Journal 6(1994): 941–948.

Itoh A, T Kumashiro, M Yamaguchi, N Nagakura, Y Mizushina,T Nishi and T Tanahashi. “Indole alkaloids and other constituents of Rauwolfia serpentina.” Journal of National Proceeding 68(2005): 848-852.

Jawahar M, A V P Kartikeyan, D Vijai, M Mahajan, S Ravipaul and M Jeyseelan. “In vitro plant regeneration from different explants of Cardiospermum helicacabum L.” International Journal of Biological and Chemical Sciences 2(2008):14-20.

Karuppusamy S and T Pullaiah. “In vitro shoot multiplication of Bupleurum distichophyllum Wight. A native medicinal plant of southern India.” Plant Tissue. Culture & Biotechnology 17(2007): 115-124.

Kirillova N V, M G Smirnova and V P Komov. “Sequential isolation of superoxide dismutase and ajmaline from tissue culture of Rauwolfia serpentina Benth.” Prikladnaia Biokhimiia Microbiologiia, 32(2001):181-185.

Kirtikar K R and B D Basu. “Indian medicinal plants” Bishen Singh Mahendra Pal Singh, Publishers, Dehradun, India 2(1993), pp.289.

Maheshwari P, B Songora,S Kumar, P Jain, K Srivastava and A Kumar. “Alkaloid production in Vernonia cinerea: callus, cell suspension and root cultures.” Journal of Biotechnology 2(2007): 1026-1032.

Mallon R, R J Oubina and M Gonzalez. “In vitro propagation of the endangered plant Centaurea ultreiae: assessment of genetic stability by cytological studies, flow cytometry and RAPD analysis.” Plant Cell Tissue and Organ Culture 101(2010): 31-39.

Mathur A, P S Ahuja and A K Mathur “Micropropagation of Panax quiquefolium, Rauwolfia serpentina and some other medicinal and aromatic Plants of India.” In: Adapted Propagation Techniques for Commercial Crops of the Tropics. (eds. Quynh N T and N V Hyen) Agriculture Publishing House, 1993, Hochi Minh, Vietnam.

Mitra G C, R N Prasad and A R Chowdhury. “Inorganic salts and differentiation of protocorms in seed callus of an orchid and correlated changes in its free amino acid content.” Indian Journal of Experimental Biology 14(1976): 350–351.

Murashige T, M Serpa and J B Jones. “Clonal multiplication of Gerbera through tissue culture.” Horticultural Science 2(1974): 170-180.

Nagatani N, S Takumi, M Tomiyama, T Shimada and E Tamiya. “Semi-real time imaging of the expression of a maize polyubiquitin promoter-GFP gene in transgenic rice.” Plant Sciences 124(1997): 49-56.

Nakasha J J, U R Sinniah, N Kemat and K S Mallappa. “Induction, subculture cycle, and regeneration of callus in safed musli (Chlorophytum borivilianum) using different types of phytohormones.” Pharmacognosy Magazine 12(2016): 460–464.

Pathania S, V Randhawa and G Bagler. “Prospecting for novel plant-derived molecules of Rauvolfia serpentina as inhibitors of aldose reductase, a potent drug target for diabetes and its complications.” PLoS ONE 8(2013): 613-627.

Prem D, I Kazutaka and T Sanro. “Stimulation of the production of podophyllotoxin by biogenetic precursors and an elicitor in Juniperus chinensis stem-derived callus cultures.” Pakistan Journal of Biological Sciences 5(2002): 313-316.

Rashmi R and M P Trivedi. “Rapid in vitro regeneration of an endangered medicinal plant sarpagandha (Rauwolfia serpentina L.)” European Journal of Pharmaceutical and Medical Research 3(2016): 276-284.

Roja G and M R Heble. “Indole alkaloids in clonal propagales of Rauwolfia serpentina Benth. Ex. Kurz.” Plant Cell Tissue and Organ Culture 44(1996): 111-115.

Roy S K, M Z Hossain and M S Islam. “Mass propagation of Rauwolfia serpentina by in vitro shoot tip culture” Plant Tissue Culture 4(1994): 69-75.

Sahoo Y and P K Chand. “Micropropagation of Vitex negundo L. a woody aromatic medicinal shrub through high frequency of axillary shoot proliferation.” Plant Cell Reports 18(1998): 301-307.

Salma U, M S M Rahman, S Islam, N Haque, M Khatun, T A Jubair and B C Paul. “Mass Propagation of Rauwolfia serpentina L. Benth.” Pakistan Journal of Biological Sciences 11(2008): 1273-1277.

Sarker K P, A Islam, R Islam, A Hoque and O I Joarder. “In vitro propagation of Rauwolfia serpentina through tissue culture.” Planta Medica 62(1996):358-359.

Shah M I, M Jabeen and I IIahi. “In vitro callus induction, its proliferation and regeneration in seed explants of wheat (Triticum vulgare L.) var. Lu-26S.” Pakistan Journal of Botany 35(2003): 209-217.

Sharma N, K P S Chandel and V K Srivastava. “In vitro propagation of Coleus forskohlii Briq. – a threatened medicinal plant.” Plant Cell Reports 10(1991): 67-70.

Smith, R H. “Plant Tissue Culture Techniques and Experiments.” Academic Press, INC., California. 1992, pp. 171.

Stanford J L, E J Martin, L A Brintin and R N Hoover. “Rauwolfia use and breast cancer. A case-control study.” Journal of National Cancer Institute 76 (1986):817-822.

Sudha CG and S Seeni. “In vitro multiplication of Adhatoda beddomei C.B. Clarke- A rare medicinal plant.” Plant Cell Reports 13(1994): 2003-2007.

Sudha C G and S Seeni. “In vitro propagation of Rauwolfia serpentina, a rare medicinal plant.” Plant Cell Tissue and Organ Culture 44(1996): 243-248.

Von Poser G, H H Andrade, K V Da Silva and J A Henriques. “Genotoxic, mutagenic and recombinogenic effects of Rauwolfia alkaloids.” Mutation Research 232(1990): 37-43.

Yamamoto O and Y Yamada. “Production of reserpine and its optimization in cultured Rauwolfia serpantina cells.” Plant Cell Reports 5(1986): 50-53.

Yoshimatsu K and K Shimomura. “Efficient shoot formation on internodal segments and alkaloid formation in the regenerates of Cephaelisipeca cuanha A. Richard.” Plant Cell Reports 9(1991): 567-570.




DOI: https://doi.org/10.21746/aps.2018.7.2.1

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