In vitro flower induction and multiple shoot regeneration studies in Solanum americanum L. (solanaceae)

Ramar K.*, ArulPrakash T., Ayyadurai V.


In vitro regeneration of Solanum americanum was achieved from explants like leaf and nodal Segments on MS medium with B5 vitamins supplemented with different concentrations and combinations of plant growth regulators. Multiple shoot induction was achieved from nodal explants cultured on MS medium supplemented with BAP 3.0 mg/L+2, 4-D 0.5 mg/L+GA3 2.0 mg/L. In vitro flower induction and multiple shoot induction was observed from leaf explants  on MS medium combination with BAP 2.0 mg/L+2,4-D 1.0mg/L+IAA 1.0 mg/L. The regenerated shoots were transferred in to MS medium fortified with NAA, IBA and IAA for root induction. Soil, compost and sand (1:1:1) mixture was the most suitable planting substrate for hardening. Regenerated plants were successfully acclimatized and survived under ex vitro conditions.


In vitro flowering; Benzyl amino purine (BAP); 2, 4-Dichlorophenoxyacetic acid (2, 4-D); Gibberellin (GA3); Indole acetic acid (IAA); Indole butyric acid (IBA); Naphthalene acetic acid.

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