In vitro flower induction and multiple shoot regeneration studies in Solanum americanum L. (solanaceae)

Ramar K.*, ArulPrakash T., Ayyadurai V.

Abstract


In vitro regeneration of Solanum americanum was achieved from explants like leaf and nodal Segments on MS medium with B5 vitamins supplemented with different concentrations and combinations of plant growth regulators. Multiple shoot induction was achieved from nodal explants cultured on MS medium supplemented with BAP 3.0 mg/L+2, 4-D 0.5 mg/L+GA3 2.0 mg/L. In vitro flower induction and multiple shoot induction was observed from leaf explants  on MS medium combination with BAP 2.0 mg/L+2,4-D 1.0mg/L+IAA 1.0 mg/L. The regenerated shoots were transferred in to MS medium fortified with NAA, IBA and IAA for root induction. Soil, compost and sand (1:1:1) mixture was the most suitable planting substrate for hardening. Regenerated plants were successfully acclimatized and survived under ex vitro conditions.

Keywords


In vitro flowering; Benzyl amino purine (BAP); 2, 4-Dichlorophenoxyacetic acid (2, 4-D); Gibberellin (GA3); Indole acetic acid (IAA); Indole butyric acid (IBA); Naphthalene acetic acid.

Full Text:

PDF

References


"Factsheet – Solanum americanum". Electronic Flora of South Australia. South Australian Government. Retrieved 29 May 2013.

Abdul Manaf Ali I, Muhammad Mukram Mackeen, Saleh H, Ei-Sharka Junainah A, Hamidi, Nor Hadiani Ismaili, Faujan BH, Ahmadi, Nordin H, Lajisi, Antiviral and Cytotoxic Activities of Some Plants Used in Malaysian Indigenous Medicine, Pertanika J. Trop. Agric. Sci, 1996, 19(2/3), 129-136.

Abdulaziz, M and Al-Bahrany, Effect of Phytohormones on in vitro shoot multiplication and rooting of lime Citrus aurantifolia (Christm.) Swing, Scientia Horticulturae, 2002, 95(4), 285–295.

Ajithkumar, D and Seeni S, Rapid clonal multiplication through in vitro axillary shoot proliferation of Aegle marmelos (L.) a medicinal tree, Plant Cell Reports, 1998, 17(5), 422-426.

Al Chami L, Mendez R, Chataing B, O'Callaghan J, Usubilliga A, Lacruz L, "Toxicological effects of α-solamargine in experimental animals" (Citation and abstract only), Phytotherapy Research , 2003, 17 (3), 254–258, Retrieved 29 May 2013.

Azad MAK, Yokota S, Ohkubo T, Andoh Y, Yahara S and Yoshizawa N, In vitro regeneration of the medicinal woody plant Philodendron amurense Rupr, through excised leaves, Plant Cell, Tissue and Organ Culture, 2005, 80, 43–50.

Conn, Barry J, (2001), "Solanum americanum – New South Wales Flora Online", Plant NET – The Plant Information Network System, 2.0. Sydney, Australia, The Royal Botanic Gardens and Domain Trust, Retrieved 29 May 2013.

Edmonds JE, Chweya JA, Black nightshades, Solanum nigrum L. and related species, International Plant Genetic Resources Institute, 1997, 66.

Engin Tilkat, Ahmet Onay, Hakan Yıldırım and Emine Ayaz, Direct plant regeneration from mature leaf explants of pistachio, Pistacia Vera L. Scientia Horticulturae, 2009, 121,361–365.

Gamborg OL, Miller RA and Ojima O, Nutrient requirements of suspension cultures of soybean root cell, Exp. Cell Res, 1968, 50, 151-158.

George EF, Sherrington PD, In: Plant Propagation by Tissue Culture, Exegetics Ltd, Eversley, England, 1984, 39–71.

Gugulothu V, Ajmeera R, Vatsvaya SR, Screening for in vitro antimicrobial activity of Solanum americanum Miller, Journal of Recent Advances in Applied Sciences, 2011, 26, 43-46.

Hyland BPM., Whiffin T, Zich, F. A. et al, (Dec 2010), "Factsheet – Solanum americanum", Australian Tropical Rainforest Plants, Edition 6.1, online version [RFK 6.1], Cairns, Australia: Commonwealth Scientific and Industrial Research Organisation, through its Division of Plant Industry, the Centre for Australian National Biodiversity Research, the Australian Tropical Herbarium, James Cook University, Retrieved 29 May 2013.

Koroch A, Juliani HR, Kapteyn J and Simon JE, In vitro regeneration of Echinacea purpurea from leaf explants, Plant Cell, Tissue and Organ Culture, 2002, 69, 79–83.

Mohy-ud-dint A, Khan Z, Ahmad M, Kashmiri MA, Chemotaxonomic value of alkaloids in Solanum nigrum complex, Pakistan Journal of Botany, 2006, 42 (1), 653–660. Retrieved 29 May 2013.

Murashige T and Skoog F, A revised medium for rapid growth and bioassays with Tobacco tissue cultures, Physiol Plant, 1962, 15(3), 473-497.

Nellis D, Poisonous Plants and Animals of Florida and the Caribbean, Pineapple Press, 1997.

R. Scorza, In vitro flowering, Hort. Rev. 4 (1982) 106–127.

Shimomura K, Yoshimatsu K, Jaziri M, Ishimaru K, Traditional medicinal plant genetic resources and biotechnology applications, Plant Biotechnology and Plant Genetic resources for Sustainability and Productivity, (Series: Biotechnology Intelligence Unit), 1997, pp. 209–25.

Sudhakaran S and Sivasankari V, In vitro flowering response of Oscimum basilicum (L.), J. Plant Biotechnol, 2002, 4(4), 181 – 183.

Vadewale AV, Barve DM, and Dave, AM, In vitro flowering and rapid propagation of Vitex negundo (L.) A medicinal plant, Indian Journal of Biotechnology, 2006, 5,112-116.

Yasin JN, Flora of Pakistan, Vol.168, Pakistan Agricultural Research Council, Islamabad, 1985, Pakistan.

Zubaida Y, Azbta KS, Syeda MA, Medicinally Important Flora of Dhibbia Karsal Village (Mianwali District Punjab), Asian Journal of Plant Sciences, 2004, 3(6), 757-762.






Copyright (c) 2014 Annals of Plant Sciences

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.