Effect of combination of different hormones on Micro propagation of Mentha sps.

Ajai K. Sharan*, Shri Rangjee Dubey, Shweta Kumari, Vikash Chandra, Bhola P. Singh, Gopal Kumar

Abstract


Adventitious bud regeneration from inter node explants of Mentha sps was achieved, shoots from nodal segments grown in vitro, were cut in to pieces and used as source of explants. Organogenesis was induced from inter nodal explants cultured on Murashige and Skoog (MS) medium containing different combinations of NAA (1mg/L) and BAP (2mg/L, NAA (1mg/L) and Kinetin (1mg/L) and Kinetin (mg/L) and IBA(1mg/L) under 16 hr photoperiod and at a temperature of 25o ±2. The types of explants markedly influenced organogenesis and growth of the regenerated shoots. The regeneration frequencies were high in internodal explants. A combination of NAA and BAP could influence the best result as it supported elongation of the shoot, multi shooting, root development and callus development of diverse nature. From the study it can be proposed that a low concentration of NAA (1mg/L) and BAP (2mg/L) can be effectively used for micro propagation of Mentha sps. The combination of NAA (1mg/L) and Kinetin (1mg/L) and Kinetin (1mg/L) and IBA (1mg/L) does not seem to induce comparable nature of effect as exhibited by combination of NAA and BAP.

Keywords


Ex plant of wild Mentha sps; Different combination of PGR; Suitability of NAA and BAP.

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