Induction of apoptosis and inhibitory potential of the methanol extract of Caesalpinia bonduc (L.) Roxb against breast cancer

Preeja G. Pillai*, Suresh P., Gitanjali Mishra

Abstract


Caesalpinia bonduc (L.) Roxb seed contains significant amounts of polyphenolic compounds that exhibit powerful antioxidant activity. The purpose of this study was to evaluate the cytotoxicity and apoptotic activity of Caesalpinia bonduc (L.) Roxb seed extract in a human breast cancer cell line (MCF-7) and antitumor activity against C3H (Jax) induced mammary cancer in C3H/Jax mice. Methanol extract of seeds of Caesalpinia bonduc (L.) Roxb was tested for cytotoxic properties on a human breast cancer cell line (MCF-7) by sulforhodamine B assay. Various doses of methanol extract of Caesalpinia bonduc (L.) Roxb (MECB) were further evaluated for induction of apoptosis in MCF-7 cells and evaluated for its inhibitory potential against C3H (Jax) induced mammary cancer in C3H/Jax mice. Methanol extract of Caesalpinia bonduc (L.) Roxb seed showed 78.4% growth inhibition against human breast cancer cells. The IC50 value of extract was 19 ± 1.4 μg/ml. Plant extract of Caesalpinia bonduc (L.) Roxb was observed to induce apoptosis of MCF-7 cells as evidenced by Sulforhodamine B dye (SRB) assay, cell-morphological changes and chromatin condensation. MECB produced significant tumor suppression during the entire duration of the study in C3H (Jax) induced tumor. Mean survival time and percentage life span also increased in MECB treated group as compared to control. Histopathology of mammary tumor showed suppressed expression of neovascularization in mammary tumor. MECB exert its chemo preventive activity may be due to antiangiogenic and apoptotic effect.

Keywords


MCF-7 cells; Breast cancer; Apoptosis; C3H (Jax) tumor

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