Utilization Of SSR Markers For Identification Of Horse Gram (Macrotyloma uniflorum (Lam.) Genotypes

Abstract

Horse gram is the poor man's pulse crop in southern India where the seeds are parched and then eaten after boiling or frying, either whole or as a meal. Horse gram (Macrotyloma uniflorum) varietal characterization and identification of distinguishing characters is most important in agricultural crops to assess genetic purity of seed in seed production programme. Characterization of twenty two horse gram genotypes viz., AK-38, AK-42,AK-42C, BGM-1, BJPL-2, CRHG-4, CRHG-17, CRHG-18, HG-07, HG-34, PHG-9C, VLG-22, VLG-21, TCR-81, TCR-73, TCR-117, TCR-223, TCR-244, TCR-268, TCR-614, TCR-74 and PHG-9 was carried out with different SSR markers of the runner bean or scarlet runner bean (Phaseolus coccineus), which will be useful in broad classification of genotypes. A set of 10 SSR primers amplified 26 alleles from 22 horse gram genotypes. On an average of 2.6 alleles per marker were amplified. All most all the primers amplified minimum of 2 alleles. Some of the primers viz., CA-910489, CA-911990, CA-911600 and CA-912170 amplified maximum of 3 alleles. Highly distinct genotypes among the selected germplasm were BGM-1 with TCR-74 and AK-38 followed by TCR-74 with TCR-614 and VLG-22 with TCR-223 which could be successfully utilized in the breeding programme and genetic purity testing of horse gram crop improvement.