Antioxidant potential of guava leaves extracts and their effects on hyperlipidemia

Abstract

The present study was carried out to check out the effect of guava leaves extract on hyperlipidemia. Guava leaves were taken from different orchards of Faisalabad after this mineral and proximate analysis were performed. Three varieties of guava were taken and extract of these three varieties were prepared with different solvents. Among these extracts one of the best variety extract was given to the rats for a period of 30 days. Blood samples were taken at the start, middle and at the end of the study to analysis the level of cholesterol. The maximum effect on the value of glutathione in hypercholestrolemic rats was seen with pink guava leaves extract in S5 the value of glutathione at 0 day was 41.59 ± 1.73 and it increased up to 44.55 ± 2.94 at 30^th day. The maximum effect on the value of TBARS in hypercholestrolemic rats was seen with pink guava leaves extract in S5 the value of TBARS at 0 day was 5.58 ± 0.52 and it increased to 6.31 ± 0.87 at 30^th day. The maximum effect on the value of urea in hypercholestrolemic rats was seen with pink guava leaves extract in S5 the value of urea at 0 day was 26.45 ± 1.74 and it decreased to 25.54 ± 0.79 at 30^th day. Maximum effective results were seen with the ethanolic extract of pink guava leaves the values at 0 day was 0.06 ± 0.02 while it decreased to 0.14 ± 0.03 at 30^th day. Least effect on the creatinine was seen with the surahi guava leaves extract in S4 group the value at 0-day was0.96 ± 0.03 while at 30^th day it decreases to 0.90 ± 0.03. Mean value of ALT was high in those rats were given pink guava leaves extract as compared to gola extract followed by extract of surahi leaves extract. Mean value of AST was high in those rats were given pink guava leaves extract as compared to gola extract followed by extract of surahi leaves extract. The mean value of DPPH was highest with ethanolic extract of pink guava leaves and its mean value was 66.87 ± 2.67 it was followed by ethanolic extract of gola guava leaves and its value was60.29 ± 3.11 and third best extraction was with ethanolic extract of Surahi leaves and its mean value was 56.75 ± 3.44 on the base of varieties. The mean value of FRAPP was highest with ethanolic extract of pink guava leaves and its mean value was 91.55 ± 2.85 it was followed by ethanolic extract of gola guava leaves and its value was 85.78 ± 1.72and third best extraction was with ethanolic extract of Surahi leaves and its mean value was 80.54 ± 3.05 on the base of varieties. Mean value of TG was minimum in normal rats that was 120.33, value of TG in hyperlipidemic rats was highest that was 164.44, value of TG in rats those were given gola leaves extract its value was 178.15 mg/dl, mean value of TG was 194.85 in rats those were given surahi leaves extract and mean value of TG in rats was 174.85mg/dl in rats those were given extract of pink guava leaves so value of TG was minimum in those rats that were given extract of pink guava leaves so extract of pink guava leaves was best among the gola and surahi. Mean value of cholesterol was minimum in normal rats that was 100.33, value of cholesterol in hyperlipidemic rats was highest that was 218.47mg/dl, value of cholesterol in rats those were given gola leaves extract its value was 202.88 mg/dl, mean value of cholesterol was 232.94 mg/dl in rats those were given surahi leaves extract and mean value of cholesterol in rats was 200.22mg/dl in rats those were given extract of pink guava leaves so value of cholesterol was minimum in those rats that were given extract of pink guava leaves so extract of pink guava leaves was best among the gola and surahi leaves extract. Mean value of LDL was minimum in normal rats that was 47.33, value of LDL in hyperlipidemic rats was highest that was 148.47, value of LDL in rats those were given gola leaves extract its value was 89.88 mg/dl, mean value of LDL was 128.44 mg/dl in rats those were given surahi leaves extract and mean value of LDL in rats was 86.22mg/dl in rats those were given extract of pink guava leaves so value of LDL was minimum in those rats that were given extract of pink guava leaves so extract of pink guava leaves was best among the gola and surahi. The mean value of HDL in rats was 35.52mg/dl in rats those were given extract of pink guava leaves so highest value of HDL was highest in those rats that were given extract of pink guava leaves so extract of pink guava leaves was best among the gola and surahi.