Cover Image

Betaine Aldehyde Dehydrogenase (BADH) gene and free amino acid analysis in Rhizophora mucronata Lam. from Thalassery region of Kerala, India.

Anusha Sreeshan, Meera S.P., Anu Augustine

Abstract


Mangroves are salt tolerant plants inhabiting saline environment. Multiple factors contribute to their salt tolerance and we need multifaceted approach to reveal the mechanisms of salt tolerance in the plant. In the present study, leaves of the mangrove, Rhizophora mucronata grown in the presence and absence of salt were used, free amino acids and the expression of selected genes were analyzed. Chromatographic technique showed the accumulation of free amino acids like proline, glycine, aspartic acid, valine, leucine and glutamic acid in the presence of salt. RNA was isolated from the leaf sample and cDNA was synthesized. Gene specific primers were designed and standardized. Among the genes studied (P5CS, BADH, NHX1), Betaine Aldehyde dehydrogenase (BADH) gene was found to be expressed.

Keywords


Amino acid, Mangrove, Salinity, Salt tolerance, Stress

Full Text:

PDF

References


Arakawa Keita, Katayama M, Takabe T."Levels of betaine and betaine aldehyde dehydrogenase activity in the green leaves, and etiolated leaves and roots of barley". Plant and Cell Physiology 31(1990): 797–803.

Farah Al Nasir, Mufeed Batarseh, Adel H. Abdel-Ghani, Anwar Jiries."Free Amino Acids Content in Some Halophytes under Salinity Stress in Arid Environment, Jordan" .Clean – Soil, Air, Water 38 (7) (2010): 592–600

Fitzgerald T L, D L E Waters, R J Henry."Betaine aldehyde dehydrogenase in plants" .Plant Biology 11(2009):119–130

Hibino, Takashi,, Meng YL, Kawamitsu Y, Uehara N, Matsuda N, Tanaka Y, Ishikawa H, Baba S, Takabe T, Wada K et al., ."Molecular cloning and functional characterization of two kinds of betaine-aldehyde dehydrogenase in betaine-accumulating mangrove Avicennia marina (Forsk.) Vierh". Plant Mol Biol 45(2001): 353–363.

Ishitani, Manabu, Toshihide Nakamura ,Seung Youn Han, Tetsuko Takabe "Expression of the betaine aldehyde dehydrogenase gene in barley in response to osmotic stress and abscisic acid". Plant Mol Biol. 27(2) (1995):307-15.

Jia, Guodong, Zhiqing Zhu, FQ Chang, YX Li."Transformation of tomato with the BADH gene from Atriplex improves salt tolerance". Plant Cell Reports 21(2) (2002): 141–146.

Joshi Govind, Thomas Dolan, Robert Gee, and Paul Saltman. "Sodium chloride effect on dark fixation of CO2 by marine & terrestrial plants". Plant Physiol 37(1962):446–449.

Liang Z , Ma D, Tang L, Hong Y, Luo A, Zhou J, Dai X." Expression of the spinach betaine aldehyde dehydrogenase (BADH) gene in transgenic tobacco plants". Chin J Biotechnol. 13(3) (1997):153-9.

Liang, Wenji, Xiaoli Ma, Peng Wan c, Lianyin Liu."Plant salt-tolerance mechanism: A review". Biochemical and Biophysical Research Communications xxx (2017) 1-6.

Meng, Xiaoqian, Jun Zhou, Na Sui.”Mechanisms of salt tolerance in halophytes: current understanding and recent advances". Open Life Sci.13 (2018): 149–154.

Mishra, Avinash and Bhakti Tanna.”Halophytes: Potential Resources for Salt Stress Tolerance Genes and Promoters". Frontiers in Plant Science 8(2017):829.

Moghaieb E A Reda, Hirofumi Saneoka, Kounosuke Fujita."Effect of salinity on osmotic adjustment, glycine betaine accumulation and the betaine aldehyde dehydrogenase gene expression in two halophytic plants, Salicornia europaea and Suaeda maritima". Plant Science 166(2004):1345–1349.

Nakamura T, Yokota S, Muramoto Y, Tsutsui K, Oguri Y, Fukui K, Takabe T ."Expression of a betaine aldehyde dehydrogenase gene in rice, a glycine betaine non-accumulator, and possible localization of its protein in peroxisomes". Plant J 11(5) (1997):1115-20.

Negrao S, Schmockel S M, Tester M."Evaluating physiological responses of plants to salinity stress". Annals of Botany 119(2017): 1–11

Parida, Asish Kumar and Bhavanath Jha. "Salt tolerance mechanisms in mangroves: a review". Trees 24(2010):199– 217.

Park, Hee Jin, Woe-Yeon Kim, and Dae-Jin Yun."A New Insight of Salt Stress Signaling in Plant. Mol. Cells 39(6) (2016): 447-459.

Rubio-Pina A Jorge and Omar Zapata-Perez."Isolation of total RNA from tissues rich in polyphenols and polysaccharides of mangrove plants". Electronic Journal of Biotechnology 14(5) (2011).

Rudulier Le Daniel, Arne R Strom, A M Dandekar, L T Smith, R C Valentine."Molecular biology of osmoregulation". Science 224(1984):1064–1068.

Shan, Liang, Zhou RenChao, Dong SuiSui1, Shi SuHua." Adaptation to salinity in mangroves: Implication on the evolution of salt-tolerance". Chinese Science Bulletin 53(11) (2008):1708-1715.

Shrestha K N, Waters D LE, Rice N." BADH genes in wheat and their expression", paper presented to the Plant and Animal Genome XIX Conference, San Diego, CA, 15-19(2011).

S P Meera, Anusha S, Anu A."Functional screening and genetic engineering of mangrove salt responsive genes: A Review". Annals of Plant Sciences 2(2013): 535-542.

Sreeshan, Anusha, Meera S P, Anu A."A review on transporters in salt tolerant mangroves". Trees 28 (2014): 957–960.

Stewart G R, Larher F."Accumulation of Amino acids and Related Compounds in Relation to Environmental Stress". In: The Biochemistry of Plants (1980) 609-635.

Weigel P, Weretilnyk E A, Hanson A D.”Betaine aldehyde oxidation by spinach chloroplasts". Plant Physiology 82(1986): 753–759.

Zhang, Ning, Huai Jun Si, Gang Wen, Hong Hui Du, Bai Lin Liu, Di Wang. "Enhanced drought and salinity tolerance in transgenic potato plants with a BADH gene from spinach". Plant Biotechnol Rep 5(2011):71–77.

Zhang Y, Yin H, Li D, Zhu W, Li Q.”Functional analysis of BADH gene promoter from Suaeda liaotungensis K". Plant Cell Rep. 27(3) (2008):585-92.




DOI: https://doi.org/10.21746/aps.2018.7.10.1

Refbacks

  • There are currently no refbacks.




Copyright (c) 2018 Annals of Plant Sciences

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.