Evaluation of Cardioprotective potential of Methanolic Extracts and its Fractions of Allium humile Leaves
Abstract
The present study has been designed to investigate the efficacy of Allium humile leaves active extract and its fraction on ischaemia and reperfusion-induced myocardial injury. Petroleum ether, chloroform, acetone and methanolic extracts of Allium humile leaves were prepared and screened for their cardio protective potential. Among all the extracts methanolic extract attenuated myocardial injury. The methanolic extract was further purified using column chromatographic technique that results four major fractions viz F1, F2, F3 and F4. These fractions were again screened for myocardial infarct size, LDH and CK-MB release in coronary effluent at dose level of 100mg/kg body weight and compared with standard drug Ramipril (1mg/kg body weight). The fraction F4 of methanolic extract significantly prevented myocardial infarct size, LDH and CK-MB release.
Keywords
Ischaemia; Reperfusion; Allium humile; Ramipril; Chromatoghraphy
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