Cover Image

Effect of chemical mutagens on chromosomal behavior of Allium cepa L.

Jyotirmoy Sharma, Jinu Devi Rajkumari, Ingtipi W., Rakesh Boro, Shivani Das

Abstract


Effect of chemical mutagens was studied in mitotic cells of Allium cepa L. For this purpose, root tips were treated with saturated aqueous solution of paradichlorobenzene, 0.2% EMS and 0.2 % freshly prepared aqueous colchicines solution for 4 hours to 9 hours. The study revealed a wide range of chromosomal abnormalities such as stickiness, laggards, chromosomal bridge, unequal separation, nuclear budding, multinuclear cells etc. The chromosomal abnormalities in different mitotic stages were calculated on mitotic index, frequency of phases and percentage of abnormalities in mitosis. The chromosomal abnormalities increase with increasing duration of treatment. The results showed that in 0.2% colchicine and 0.2%EMS treated cells more than 50% abnormalities were observed after 9 hrs of treatment. While in PDB maximum chromosomal abnormalities observed after 6hrs of treatment. Maximum MI was recorded as 68% in controlled cells. While in PDB, colchicines and EMS treated cells minimum 9.5%, 20% and 23% MI were observed respectively after 9hrs of treatment.


Keywords


Chromosome, Chromosome aberration, Mitotic index, Mutagens

Full Text:

PDF

References


Doroftei E, Bercu. R: The cytotoxic effects and mutagen potential induced by sulphites at Allium cepa L. Annals of RSCB, 2013, XVIII (I)

Hajra NG: Induction of mutations by chemical mutagens in tall indica rice. Indian Agric,, 1979,23, 67-72

Hilada N, Jasmin M, Azra M, and Kemajl K.: Chromosomal and Nuclear Alterations in Root Tip Cells of Allium Cepa L. Induced by Alprazola chemosphere.2017.11, 101

Joshi N, Ravindran A and Mahajan V: Investigations on Chemical Mutagen Sensitivity in 4. Onion (Allium cepa L.: International Journal of Botany, 2011, 7 (3), 243-248.

Natarajan AT: A cytogenetical study of the effect of mutagen on plant with special reference to the induction. Ph.D. Thesis, 1958, IARI, New Delhi

Ramesh B: Comparison of cytological effects of EMS on dry and presoaked seeds of rice variety IR8.Plant and Nature, 1983,1, 39-43

Reddi V.R.and Reddi TVVS: Cytogenetical effect of chemical mutagen in rice, Cytologia,1984, 49

Roy RP, Sinha BMB and Thakur GK: Irradiation studies in Cucumis sativus L., J. Cytol Genet, 1971, 6,128-135

Sharma AK and Sharma A.: Chromosome Technique & Practice.Bullerwort, London. 1980

Siddiq IA: Studieson induction of polyploidy in maize and Sorghum and on the elimination of diploid cells in colchicines treated maize, 1964, Ph. D Thesis IARI Delhi

Thurling N and Depittayanan, V.: EMS induction of early flowering mutants in springrape (Brassica napus) Pl. Breed, 1992, 108, 177 – 184.

Vadluri R, MuraliKrishna. T, Jogam P.and Sadanandam A.: Evaluation of morphological and cytological variations induced by ethyl methane sulphonate in Capsicum annum L. Int. J. Adv. Res., 2016, 11, 2398-2400.




DOI: https://doi.org/10.21746/aps.2018.7.4.22



Copyright (c) 2018 Annals of Plant Sciences

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.