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HPLC identification of bioactive flavonoids in the methanolic and aqueous leaf extracts of Homalium zeylanicum Benth.

Anuradha Devi V., Veera Kishore I., Ragha Latha R., Kokkanti Mallikarjuna

Abstract


Natural products continue to provide unique structural diversity in comparison to standard combinatorial chemistry, which presents opportunities for discovering mainly novel low molecular weight lead compounds. Homalium zeylanicum belonging to the family Flacourtiaceae is an important medicinal plant having traditional uses in diabetes, rheumatism and wound healing activities. Chromatographic methods like TLC and HPLC were used for the separation and identification of flavonoids present in methanolic and aqueous leaf extract was studied. The chromatographic methods available for the separation of flavonoids in TLC and HPLC were adopted for the study. Three compounds were identified in TLC study in methanolic leaf extracts. In HPLC analysis, peaks corresponding to flavonoids were obtained and were identified by comparing with literature and confirm that methanolic extract contains Rutin, Quercetin and Myricetin where as in aqueous extract Quercetin, Myricetin and Kaempferol were observed. The anti diabetic activity of isolated compounds was determined by literature and confirms that compounds were found to be having potent anti diabetic activity. This proves that the anti-diabetic activity of Homalium zeylanicum was due to the presence of these bio-active compounds.

Keywords


Homalium zeylanicum, flavonoids, the anti-diabetic activity, TLC, HPLC.

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References


Aguirre L, Noemi Arias, M. Teresa Macarulla, Ana Gracia and Maria P. Portillo. “Beneficial effects of quaercetin on obesity and diabetes.” The open nutraceuticals journal. 4, (2011):189-198.

Anuradha Devi. V and K. Mallikarjuna. “In vitro antimicrobial and antidiabetic activity of leaf Extracts of Schrebera swietenioides and Homalium zeylanicum”. International Journal of life science and Pharma research, 6(3) (2016): 1 – 7.

Alvarado A.J., Mauricio Peñarrieta J, Bjorn Akesson and Bjorn Bergenståhl, “Separation of phenolic compounds from foods by Reversed-phase high performance liquid Chromatography”. Revista boliviana de química. 24(1) (2007): 1-4.

Bosser J, Rabevohitra R. “The genus Scherebera found in madagascar, Bull Mus Nation hist nat paris, 4(7), (1985):59-66.

De Oliveira Bras H. de, Tomoe Nakashima, José D. de Souza Filho and Fabiano L. Frehse. “HPLC Analysis of Flavonoids in Eupatorium littorale” J. Braz. Chem. Soc., 12(2) (2001): 243-246.

El-Olemy MM, Al-Muhtadi FJ and Afifi AA. “Experimental Phytochemistry. A Laboratory Manual.” (King Saud University Press, KSA) (1994): 8-9.

Hansraj Manda. “Antioxidant, anti- Inflammatory and antipyretic activities of ethyl acetate fraction of ethanolic extract of Schrebera swietenioides”. Roxb. root, IJTPR, 1(1) (2009): 7-11.

Jadhav R and G Puchchakayala G. “Hypoglycemic and antidiabetic activity of flavonoids: boswellic acid, ellagic acid, quercetin, rutin on streptozotocin- nicotinamide induced type 2 diabetic rats”. International Journal of Pharmacy and Pharmaceutical Sciences. 4(2) (2012): 251-256.

Kang S.J., Jung-Han Yoon ParkHa-Neual and ChoiJung-In Kim. “ꟹ-glucosidase inhibitory activities of myricetin in animal models of diabetes mellitus” Food science and biotechnology.24 (5) (2015):1897-1900.

Nambiar. V. P. K. “Indian Medicinal Plants: A Compendium of 500 Species”. 5th edition, Orient Longman Private ltd, Annasalai, Chennai, (1996), pp 88-95.

Niture, Ansari and Naik. “Anti-hyperglycemic activity of rutin in streptozotocin induced diabetic rats: an effect mediated through cytokines, antioxidants and lipid biomarkers”. Indian J Exp Boil. 52(7) (2014): 720-7.

Rajkumar S. Bagali, “Evaluation of antidiabetic and antioxidant effect of Schrebera swietenioides fruit ethenolic extract”. Der Pharmacia Lettre, 2(5) (2010): 278- 288.

Rasal AS, “Evaluation of the healing potential of Schrebera swietenioides in the dexamethasone-suppressed wound healing in rodents” Int J Low Extrem Wounds., 8(3) (2009):147-52.

Sathish K. T., Baskar.R, Shanmugam.S, Rajasekaran.P, Sadasivam.S and Manikandan.V “Optimization of flavonoids extraction from the leaves of Tabernaemontana heyneana” Wall., using L16 Orthogonal design, Nature and Science. 6(3) (2008): 10-21.

Shweta yadav and padma kumar. “Production, isolation and identification of flavonoids from aerial parts of Hiptage benghalensis” international journal of life science and pharma research 2(3) (2012): 1-5.

Samariya Krishna and Sarin Renu. “Isolation and identification of flavonoids from Cyperus rotundus linn., in vivo and in vitro” Journal of Drug Delivery and Therapeutics. 3(2) (2013): 109-113.

Velavarthipati Anuradha devi and Kokkanti Mallikarjuna. “Screening and quantification of phytochemicals in leaf Extracts of Schrebera swietenioides and Homalium zeylanicum”. International Journal of Pharma and Bio Sciences, 6(4) (2015): 521 – 529.

Vessal M, Hemmati M and Vasei M. “Antidiabetic effects of quercetin in streptozocin-induced diabetic rats” Comp Biochem Physiol C Toxicol Pharmacol. 135(3) (2003):357-64.

Zang Y, Sato H, Igarashi K. “Anti-diabetic effects of a kaempferol glycoside-rich fraction from unripe soybean (Edamame, Glycine max L. Merrill. ‘Jindai’) leaves on KK-A(Y) mice”. Bio Sci Biotechnol Biochem.75 (9) (2011):1677-84




DOI: https://doi.org/10.21746/aps.2018.7.4.5



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