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In vitro conservation of rare, medicinally important species Dendrobum nobile Lindl. (Orchidaceae)

Saranjeet Kaur

Abstract


A comparative study was made to access the regeneration response of the stem-node discs of Dendrobium nobile procured from in vivo (greenhouse) grown plants and in vitro grown cultures. The response of stem-node discs, procured both from in vivo and in vitro source, was successfully tested in M (Mitra et al., 1976) medium alone and in combinations with cytokinins [N6-benzyladenine (6-BA - 0.5, 1.0 mg l-1), furfurylaminopurine (Kn - 0.5, 1.0 mg l-1) and auxin [α-naphthalene acetic acid (NAA - 0.5, 1.0 mg l-1)]. The explants regenerated via shoot bud initiation in the cultures. Juvenility of the tissues and chemical stimulus were the major factors in initiating the response in the explants. The initiation of regeneration response in in vitro derived explants was not obligatory to a treatment with growth regulators as compared to those derived from in vivo ones. Cytokinins at 1.0 mg l-1 favoured optimum regeneration percentage in the explants and invoked additional supernumerary loci in the regenerants. The regenerants from in vitro derived explants developed plantlets early in cytokinins enriched combinations.

Keywords


in vitro, micropropagation, rare, shoot buds, stem-discs, plant growth regulators

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References


Arditti J and R Ernst. “Micropropagation of orchids.” John Wiley & Sons, New York (1993), pp. 640.

Basker S and V B Narmatha. “Micropropagation of Coelogyne stricta (D. Don) Schltr. via pseudobulb segment culture.” Tropical and Subtropical Agrosystems 6(2006): 31-35.

Bensky D, X Gamble and T Kaptchuk. “Chinese herbal medicine.” Eastland Press, Seatle – Washington (1986).

Butcher D and SA Marlow. “Asymbiotic germination of epiphytic and terrestrial orchids.” In: Modern methods in orchid conservation: The role of physiology, ecology, and management (ed. Pritchard H W) Cambridge University Press, Cambridge (1989), pp. 31-38.

CITES. (2017) http://www.cites.org/eng/app/appendices.php

Decruse S W, A Gangaprasad, S Seeni and S V Menon. “Micropropagation and ecorestoration of Vanda spathulata, an exquisite orchid.” Plant Cell Tissue and Organ Culture 72(2003): 199–202.

Gangaprasad A, P G Latha, and S Seeni. “Micropropagation of terrestrial orchids, Anoectochilus sikkimensis and Anoectochilus regalis.” Indian Journal of Experimental Biology 38(2000): 149-154.

George E S. “Plant propagation by tissue culture.” Exegetics, England, 1993.

George P S and G A Ravishankar. “In vitro multiplication of Vanilla planifolia using axillary bud explants.” Plant Cell Reports 16(1997): 490–494.

Hicks A L and K Lynn.“Orchid seed germination Media. A compendium of formulations, The orchid seed bank project.” Chandler, Arizona, 2010, pp. 211.

Hong P I, J T Chen and W C Chang. “Shoot development and plant regeneration from protocorm-like bodies of Zygopetalum mackayi.” In Vitro Cellular and Developmental Biology-Plant 46(2010): 306–311.

Janarthanam B and S Seshadri. “Plantlet regeneration from leaf derived callus of Vanilla planifolia Andr.” In Vitro Cellular and Developmental Biology-Plant 44(2008): 84–89.

Kanjilal B, D De Sarkar, J Mitra, and B K Dutta. “Stem disc culture: development of a rapid mass propagation method of Dendrobium moschatum (Buch-Ham) Swartz—an endangered orchid.” Current Science 77(1999): 497–500.

Kaur Saranjeet and K K Bhutani. “Micropropagation of Malaxis acuminata D. Don: a rare orchid of high therapeutic value.” Open Access Journal of Medicinal and Aromatic Plants 1(2010): 29-33.

Kaur Saranjeet and K K Bhutani. “In Vitro mass propagation of ornamentally and medicinally important Coelogyne flaccida Lindl. Through pseudobulb segments.” Plant Tissue Culture and Biotechnology 23(2013): 39- 47.

Kaur Saranjeet and K K Bhutani. “Organic growth supplement stimulants for in vitro multiplication of Cymbidium pendulum (Roxb.). Sw.” Horticultural Science 39(2012): 47–52.

Lawler L J. “Ethnobotany of the orchidaceae.” In: Orchid Biology - Reviews and Perspectives (ed. Arditti J), Cornell University Press, Ithaca-New York 3(1984), pp.27-145.

Martin K P. “Micropropagation of the Bamboo orchid Arundina graminifolia (D. Don) HOCHR.) through protocorm-like bodies using node explants.” Propagation of Ornamental Plants 7(2007): 97–100.

Medina R D, E A Flachsland, A M Gonzalez, G Terada, M M Faloci, and L A Mroginski. “In vitro tuberization and plant regeneration from multinodal segment culture of Habenaria bractescens Lindl, an Argentinean wetland orchid.” Plant Cell Tissue and Organ Culture 97(2009): 91–101.

Mitra G C, R N Prasad and A R Chowdhury. “Inorganic salts and differentiation of protocorms in seed callus of an orchid and correlated changes in its free amino acid content.” Indian Journal of Experimental Biology 14(1976): 350-351.

Moraes L M, R T Faria and F L Cuquel. “Activated charcoal for in vitro propagation of Brazilian orchids.” Acta Horticulturae 683(2005): 383-390.

Pyati A N, H N Murthy, E J Hahn, and K Y Paek. “In vitro propagation of Dendrobium macrostachyum Lindl.-a threatened orchid.” Indian Journal of Experimental Biology 40(2002): 620–623.

Rangsayatorn N. “Micropropagation of Dendrobium draconis Rchb.f. from thin cross-section culture.” Scientia Horticulturae 122(2009): 662–665.

Rastogi S and U N Dwivedi. “Down-regulation of lignin biosynthesis in transgenic Leucaena leucocephala harboring O-methyltransferase gene.” Biotechnology Progress 22(2006): 609–616.

Terzi M and Lo Schiavo. 1990. “Somatic embryogenesis.” In: Development in Crop Science, Plant Tissue Culture: Application and Limitations (ed. Bhojwani S S), Elsevier, Amsterdam, Oxford, New York, Tokyo (1990), pp.115-151.

Vij S P and Saranjeet Kaur. Micropropagation of therapeutically important orchids: Malaxis acuminata. The Journal of the Orchid Society of India 12(1998): 89–93.

Vij S P, A Kher and P Pathak. “Micropropagation of Bulbophyllum careyanum (Hook.) Spreng. using pseudobulb segments.” The Journal of the Orchid Society of India 14(2000): 47–56.

Zhao Y, Y O Son, S S Kin, Y S Jang, and J C Lee. “Antioxidant and anti-hypoglycemic activity of polysaccharide isolated from Dendrobium chrysotoxum Lindl.” Journal of Biochemistry and Molecular Biology 40(2007): 670-677.




DOI: https://doi.org/10.21746/aps.2018.7.3.6



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