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Micropropagation of traditional medicinal plant Ceropegia juncea

Binish T.


Ceropegia species which possess wide medicinal properties are being used in different traditional medicinal systems that are used by tribal people for curing different ailments. Ceropegia juncea was reported to be the source of ‘Soma’, a plant drug of the Ayurvedic system of medicine. The plant extract is used for the treatment of anti-inflammatory, analgesic, antiulcer activities, liver disorders, hypotension, ulcerative condition and fever. It is also used as typical anesthetic agent. The present study was conducted to establish a protocol for in- vitro propagation of an endemic medicinal plant Ceropegia juncea maximum shoot proliferation better shoots with a sprouting frequency of 86% and with an average of 8.28 ±1.11 shoots /explants and attained a length of 5.37±0.74 cm was achieved on Murashige and Skoog’s, 1962 (MS) medium supplemented with 6-benzylaminopurine (BAP) 1.5 mg/L + NAA 1.0mg/L and highest rooting of in vitro derived shoots was achieved on half MS with IBA 0.75mg/L.


In vitro; Ceropegia juncea

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Britto SJ, Natarajan E and Arockiasamy DI. In vitro flowering and shoot multiplication from nodal explants of Ceropegia bulbosa Roxb. var. bulbosa. Taiwania, 48.2 (2003) pp. 106-111.

Catapan E, Moreno FN, Silva ML, Otuki MF, Niero R, Filho VC, Yermes RA and Viana AM. Protocols for In vitro Culture and Phytochemical Analysis of Phyllanthus species (Euphorbiaceae), Journal of Biomedicine and Life Science, 547.1 (2009) pp.167-177.

Chandore AN, Nimbalkar MS, Gurav RV, Bapat VA and Yadav SR. An efficient micropropagation protocol for multiplication and restoration of Ceropegia fantastica Sedgw.: a critically endangered plant species. Current Science 99 (2010) pp. 1593-1596.

Gangaprasad A, Decruse SW, Seeni S and Nair GM. Micropropagation of ecorestoration of Decalepis arayalpathra (Joseph and Chandra) Venter- An endemic and endangered ethnomedicinal Plant of Western Ghats. Indian Journal of Biotechnology, 4 (2005) pp. 265-270.

Jagtap A P and Singh N P. Fascicles of flora of India (Botanical Survey of India) Kolkatta (1999) pp. 211-241.

Karuppusamy S, Kiranmai C, Aruna V and Pullaiah T. In vitro conservation of Ceropegia intermedia –An endemic plant of South India. African Journal of Biotechnology 8.17 (2009) pp. 4052-4057.

Khosravi P, Maryam J K., Gorban A N. and Bihamta M R. A protocol for mass production of Rosa hybrid cv. Iceberg through in vitro propagation. Iranian Journal of Biotechnology 5 (2007) pp. 100-104.

Kim C K., Oh J Y, Jee So and Chung J D. In vitro micropropagation of Rosa hybrid L. Plant Biotechnology 5 (2003) pp. 115-119.

Kumar S R S, Krishna V, Venkatesh, Pradeepa K, Kumar K G and Gnanesh AU. Direct and indirect method of plant regeneration from root explants of Caesalpinia banduc (L) Roxb. A threatened medicinal plant of Western Ghats. Indian Journal of Experimental Biology 50 (2012) pp. 910-917.

Martin K P. Rapid propagation of Holostemma ada-kodium, a rare medicinal plant. Plant Cell Tissue Organ Culture 72 (2002) pp. 79-82.

Murashige T and Skoog F. A revised medium from rapid growth and bioassays with tobacco tissue cultures. Plant Physiology 15.3 (1962) pp. 473-497.

Paras S, Mehta SC, Gargee D, Lakshmayya B, Sunil K. Gastroprotective and antioxidant activities of Ceropegia juncea leaf ethanol extract. Der Pharmacia Sinica, 2.4 (2011) pp. 99-107.

Patil VM. Micropropagation of Ceropegia spp. In vitro cellular Developmental Biology- Plant 34 (1998) pp. 240-243.

Pavankumar VSPN, Vrushabendra Swamy BM, Vishwanath KM and Raja Sekhar VSP. Antioxidant and Hepatoprotective potential of Ceropegia juncea Roxb in Paracetamol induced hepatotoxicity in rats. Research Journal of Pharmaceutical Biological and Chemical Science 3.1(2012) pp. 930-944.

Pillai Geetha S, Raghu A V, Martin G, George S. and Balachandran I. In vitro Propagation of two tuberous Medicinal plants: Holostemma ada - kodiea and Ipomoea mauritiana. Journal of Biomedicine and Life Seience, 547.1 (2009) pp. 81-92.

Pradhan C, Kar S, Pattnaik S. and Chand P K. Propagation of Dalbergia sissoo Roxb. Through In vitro shoot proliferation from cotyledonary nodes. Plant Cell Reports 18 (1998) pp. 122-126.

Pullaiah T, Sandhya Rani S and Karuppusamy S. Flora of Eastern Ghats, vol.4. Regency publications Delhi. (2011) pp. 99.

Ramulu R D, Murthy K S R., Pullaiah T. In vitro Propagation of Cynanchum calliatum. Journal of Tropical Medicinal Plants 3 (2002) pp. 233-238.

Rathore M S, Dagla H R., Singh M and Shekhawat N S. Rational development of in vitro methods for conservation, propagation and characterization of Caralluma edulis. World Journal of Agricultural Science, 4.1 (2008) pp. 121-124.

Reddy P S, Gopal G R., Sita G L. In vitro multiplication of Gymnema sylvestre R. Br. An important medicinal plant. Current Science, 75 (1998) pp. 843-845.

Sukumar E, Gopal H and Rao RB. Pharmacological actions of cerpegin, a novel pyridine alkaloid from Ceropegia juncea. Fitoterapia, 66 (1995) pp. 403-406.

Tyagi P, Khanduja S and Kothari S L. In vitro Culture of Capparis deciduas and assessment of clonal fidelity of the regenerated plants. Journal of Biologia Plantarum 54.1 (2010) pp. 126-130.

Ugraiah A, Raja Sreelatha V, Krishna Reddy P V, Rajasekhar K., Sandhya Rani S, Karuppusamy S and Pullaiah T. In vitro shoot multiplication and conservation of Caralluma bhupenderiana Sarkaria-an endangered medicinal plant from South India. African Journal of Biotechnology 10.46 (2011) pp. 9328-9336.


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