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Comparison of L-Dopa content in three species of genus Mucuna by differente techniques

Ragni Vora, Ambika N. Joshi, Nitesh Chandulal Joshi

Abstract


In the present study, attempts are made to develop suitable method(s) for extraction of L-DOPA from the powdered seeds of 3 species of Mucuna using different solvents and conditions. The seed powder of both plants was subjected to 6 different extraction methods, with different solvent ratios. All the extracts were analyzed using RP-HPLC and were validated according to The International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use guidelines. The L-DOPA extraction was best with Methanol Water mixture in a cold maceration technique and overall gives good extraction efficiency in all the three plants giving concentrations of 5.03%, 13.36 % and 16.78% of L-DOPA in Mucuna gigantea, Mucuna pruriens and Mucuna monosperma, respectively. The present investigation was done to study the extraction efficiency of various extraction methods of L-DOPA content in seed extracts of Mucuna and compare it.

Keywords


Comparison, Extraction efficiency, HPLC, Intra-specific, L-DOPA, Mucuna

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DOI: https://doi.org/10.21746/aps.2018.7.1.10

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