In vitro maintenance of bioactive Ca2+ and primary metabolite status in Cissus quadrangularis under salt stress
Abstract
Cissus quadrangularis is a plant, which has been customarily used in the Indian subcontinent to hasten the process of healing in bone fracture. The production of callus tissue was achieved from mature nodal segments by culturing on MS medium supplemented with auxin and cytokinin. Addition of 50mM salt to the medium, decreased potassium and phosphorus content of callus, but increased nitrogen, sodium and calcium content. Further increase in NaCl concentration decreased phosphorus, but increased nitrogen, potassium, sodium and calcium content as compared to control (without salt). Accumulation of calcium ions at higher salt concentration may possibly help in faster remodeling of bones. Calcium ion also mitigates the adverse effects of salinity up to a certain level.
Keywords
Bioactive Ca2+; Cissus quadrangularis; NaCl; Salt overly sensitive.
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