
Estimating lipids for bioprospecting: A case study with Deepor beel algae
Abstract
Microalgae have been considered as ideal candidates for biodiesel production and other bio-products. They have received extensive research for bioprospecting during the past few decades. Chlorophycean algae are one of the few microalgae employed for many biotechnological applications. Therefore, the present work was aimed to analyze the growth and lipid contents of four Chlorophycean algae isolated from Deepor beel Ramsar site, Assam (India) to assess their potentiality for industrial means. Of the four species, Carteria cerasiformis showed the highest lipid content (24.4 ± 1.45 %).
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Ahmed F, Fanning K, Netzel M, Turner W, Li Y and Schenk PM. “Profiling of carotenoids and antioxidant capacity of microalgae from subtropical coastal and brackish waters.” Food Chem 165 (2014): 300-306.
Alkhamis Y and Qin JG. “Comparison of pigment and proximate compositions of Tisochrysis lutea in phototrophic and mixotrophic cultures.” J Appl Phycol 28 (2016): 35-42.
Bartley ML, Boeing WJ, Corcoran AA, Holguin FO and Schaub T. “Effects of salinity on growth and lipid accumulation of biofuel microalga Nannochloropsis salina and invading organisms.” Biomass Bioenerg 54 (2013): 83-88.
Chtourou H, Dahmen I, Jebali A, Karray F, Hassairi I, Abdelkafi S, Ayadi H, Sayadi S and Dhouib A. “Characterization of Amphora sp., a newly isolated diatom wild strain, potentially usable for biodiesel production.” Bioprocess Biosyst Eng 38 (2015): 1381-1392.
Del Campo JA, García-González M and Guerrero MG. “Outdoor cultivation of microalgae for carotenoid production: current state and perspectives.” Appl Microbiol Biotechnol 74 (2007): 1163–1174.
Durvasula R, Hurwitz I, Fieck A and Rao DVS. “Culture, growth, pigments and lipid content of Scenedesmus species, an extremophile microalga from Soda Dam, New Mexico in wastewater.” Algal Res 10 (2015): 128-133.
Eyster KM. “The membrane and lipids as integral participants in signal transduction: lipid signal transduction for the non-lipid biochemist.” Adv Physiol Educ 31 (2007): 5-16.
Fakhry EM and El-Maghraby DM. “Lipid accumulation in response to nitrogen limitation and variation of temperature in Nannochloropsis salina.” Bot Stud 56 (2015): 6.
Folch J, Lees M and Stanley GHS. “A simple method for the isolation and purification of total lipids from animal tissues.” J Biol Chem 226 (1957): 497-509.
Goiris K, Muylaert K, Fraeye I, Foubert I, De Brabanter J and De Cooman L. “Antioxidant potential of microalgae in relation to their phenolic and carotenoid content.” J Appl Phycol 24.6 (2012): 1477-1486.
Griffiths MJ, Garcin C, Van Hille RP and Harrison STL. “Interference by pigment in the estimation of microalgal biomass concentration by optical density.” J Microbiol Meth 85 (2011): 119-123.
Halim R, Danquah MK and Webley PA. “Extraction of oil from microalgae for biodiesel production: a review.” Biotechnol Adv 30 (2012): 709-732.
Hawati TU, Willke T and Vorlop KD. “Characterization of the lipid accumulation in a tropical freshwater microalgae Chlorococcum sp.” Bioresour Technol 121 (2012): 54-60.
Ho SH, Nakanishi A, Ye X, Chang JS, Hara K, Hasunuma T and Kondo A. “Optimizing biodiesel production in marine Chlamydomonas sp. JSC4 through metabolic profiling and an innovative salinity-gradient strategy.” Biotechnol Biofuels 7 (2014): 97.
Huerlimann R, de Nys R and Heimann K. “Growth, lipid content, productivity and fatty acid composition of tropical microalgae for scale-up production.” Biotechnol Bioeng 107 (2010): 245-257.
Kim DG and Hur SB. Growth and fatty acid composition of three heterotrophic Chlorella species.” Algae 28 (2013): 101-109.
Malcata FX. “Microalgae and biofuels: a promising partnership.” Trends Biotechnol 29 (2011): 542-549.
Matsukawa R, Hotta M, Masuda Y, Chihara M and Karube I. “Antioxidants from carbon dioxide fixing Chlorella sorokiniana.” J Appl Phycol 12 (2000): 263-267.
Milledge JJ. “Commercial application of microalgae other than as biofuels: a brief review.” Rev Environ Sci Biotechnol 10 (2011): 31-41.
Mubarak M, Shaija A and Suchithra TV. “A review on the extraction of lipid from microalgae for biodiesel production.” Algal Res 7 (2015): 117-123.
Mutanda T, Ramesh D, Karthikeyan S, Kumari S, Anandraj A and Bux F. “Bioprospecting for hyper-lipid producing microalgal strains for sustainable biofuel production.” Bioresour Technol 102 (2011): 57-70.
Ngangkham M, Ratha S, Prasanna R, Saxena A, Dhar D, Sarika C and Prasad R. “Biochemical modulation of growth, lipid quality and productivity in mixotrophic cultures of Chlorella sorokiniana.” SpringerPlus 1 (2012): 1-13.
Onay M, Sonmez C, Oktem HA and Yucel M. “Evaluation of various extraction techniques for efficient lipid recovery from thermo-resistant microalgae, Hindakia, Scenedesmus and Micractinium species-comparison of lipid extraction methods from microalgae.” Am J Analyt Chem 7 (2016): 141-150.
Orr V and Rehmann L. “Improvement of the Nile Red fluorescence assay for determination of total lipid content in microalgae independent of chlorophyll content.” J Appl Phycol 27 (2015): 2181-2189.
Patnaik R and Mallick N. “Utilization of Scenedesmus obliquus biomass as feedstock for biodiesel and other industrially important co-products: An integrated paradigm for microalgal Biorefinery.” Algal Res 12 (2015): 328-336.
Perumal GM and Anand N. “Manual of freshwater algae of Tamil Nadu.” 2009: 41-73.
Rodolfi L, Zittelli GC, Bassi N, Padovani G, Biondi N, Bonini G and Tredici MR. “Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor.” Biotechnol Bioeng 102 (2008): 100-112.
Salama ES, Kim HC, Abou-Shanab RAI, Ji MK, Oh YK, Kim SH and Jeon BH. “Biomass, lipid content and fatty acid composition of freshwater Chlamydomonas mexicana and Scenedesmus obliquus grown under salt stress.” Bioprocess Biosyst Eng 36 (2013): 827-833.
Simopoulos AP. “The importance of the ratio of omega-6/omega-3 essential fatty acids.” Biomed Pharmacother 56 (2002): 365-379.
Solana M, Rizza CS and Bertucco A. “Exploiting microalgae as a source of essential fatty acids by supercritical fluid extraction of lipids: comparison between Scenedesmus obliquus, Chlorella protothecoides and Nannochloropsis salina.” J Supercrit Fluids 92 (2014): 311-318.
Spolaore P, Joannis-Cassan C, Duran E and Isambert A. “Commercial applications of microalgae.” J Biosci Bioeng 101 (2006): 87–96.
Stranska-Zachariasova M, Kastanek P, Dzuman Z, Rubert J, Godula M and Hajslova J. “Bioprospecting of microalgae: Proper extraction followed by high performance liquid chromatographic-high resolution mass spectrometric fingerprinting as key tools for successful metabolom characterization.” J Chromatogr B 1015-1016 (2016): 22-23.
Talebi AF, Mohtashami SK, Tabatabaei M, Tohidfar M, Bagheri A, Zeinalabedini M, Hadavand-Mirzaei H, Mirzajanzadeh M, Malekzadeh-Shafaroudi S and Bakhtiari S. “Fatty acid profiling: a selective criterion for screening microalgae strains for biodiesel production.” Algal Res 2 (2013): 258-267.
Varfolomeev SD and Wasserman LA. “Microalgae as source of biofuel, food, fodder and medicines.” Appl Biochem Microbiol 47.9 (2011): 789-807.
Vello V, Phang SM, Chu WL, Majid NA, Lim PE and Loh SK. “Lipid productivity and fatty acid composition-guided selection of Chlorella strains isolated from Malaysia for biodiesel production.” J Appl Phycol 26 (2014): 1399-1413.
Zhao G, Yu J, Jiang F, Zhang X and Tan T. “The effect of different trophic modes on lipid accumulation of Scenedesmus quadricauda.” Bioresour Technol 114 (2012): 466-471.
DOI: https://doi.org/10.21746/aps.2017.6.10.6
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