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Leaf and seedling morphology: the two prospective parameters to trace phylogeny in Apiaceae subfamily Apioideae

Saubhik Das

Abstract


Determination of phylogenetic affinity among the members of Apiaceae especially the subfamily Apioideae is much debatable. The morphological evidences on inflorescence, fruit and seed characters showed little concomitance with those derived from molecular parameters as far as groupings in clades are concerned. In the present study involving few selected Apiaceae members, a Dendrogram was computed and a putative evolutionary trend was outlined based on leaf margin insertion pattern and cotyledonary leaf features, general plant morphology as well as leaf surface micromorphology by SEM. The members of Apiaceae with broad-ovate pinnatifid to pinnatipartite leaves and ovate –lanceolate cotyledonary leaves were included in a distinct cluster but the members with pinnatisect – decompounds leaves and linear cotyledonary leaves were included in a separate cluster. Long Coriander (Eryngium foetidum) included in the subfamily Saniculoideae may represent the most primitive condition having simple entire leaf with spiny margin. The member like Long coriander might have served as progenitor stock from which Coriander, Celery, Parsley (all with ovate pinnatifid to pinnatipartite leaves) and Cumin, Fennel, Dill (all having pinnatisect to decompounds leaves with linear segments) might have originated as evidenced by gradual increase in marginal insertion. Similarly, gradual narrowing of ovate-lanceolate cotyledonary leaves might have given rise to narrow linear cotyledonary leaves. Leaf-margin insertion pattern and cotyledonary leaf features appeared to be promising in tracing phylogeny in Apiaceae especially in Apioideae. Simple entire leaf represents the primitive condition and evolution in Apiaceae supposed to have progressed towards gradual increase in marginal insertion leading to pinnately dissected, pinnatipartite and ultimately to decompound leaves with narrow linear segments. Dendrogram computed from morphological features showed concomitance with that phylogenetic trend as far as grouping and interrelationships of members are concerned.  In the present study leaf margin insertion pattern and seedling morphology appeared to be instrumental in tracing the phylogeny in Apiaceae specially subfamily Apioideae while molecular systematics are not conclusive.


Keywords


Apiaceae; subfamily Apioideae; leaf-margin insertion; seedling morphology; phylogenetic affinity

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References


Baldwin, B.G., M.J. Sanderson, J.M. Porter, M.F. Wojciechowski, C.S. Campbell and M.J. Donoghue. “The ITS region of nuclear ribosomal DNA: A valuable source of evidence on angiosperm phylogeny.” Annals of Missouri Botanical Garden 82(1995):247-277. Print

Calvino, C.I., S.G. Martinez and S.R. Downie. “Morphology and biogeography of Apiaceae subfamily Saniculoideae as inferred by phylogenetic analysis of molecular data.” American Journal of Botany 96.2 (2008): 196-214. Print

Constance, L. History of classification of Umbelliferae (Apiaceae). In: Heywood, V.H., (ed), The biology and chemistry of the Unbelliferae. Academic Press, New York, 1971, pp. 1-12.

Das, S. “Systematics and taxonomic delimitation of vegetable, grain and weed amaranths: a morphological and biochemical approach.” Genetic Resources and Crop Evolution 59 (2012): 289-303. Print

Downie, S.R. and D.S. Katz-Downie. “A molecular phylogeny of Apiaceae subfamily Apioideae: evidence from nuclear ribosomal DNA internal transcribed spacer sequence.” American Journal of Botany 83 (1996): 234-251. Print

Downie, S.R., D.S. Katz-Downie and K.J. Cho. “Phylogenetic analysis of Apiaceae subfamily Apioideae using nucleotide sequence from the Chloroplast rpoCl intron.” Molecular Phylogenetics and Evolution 6(1996): 1-18.Print

Downie, S.R., S. Ramnath, D.S. Katz-Downie and E. Llanas. “Molecular systematic of Apiaceae subfamily Apioideae: phylogenetic analysis of nuclear ribosomal DNA internal transcribed spacer and plastid rpoC1 intron sequence. “American Journal of Botany 85 (1998): 563-591. Print

Downie, S.R., G.M. Plunkett, M.F. Watson, K. Spalik, D.S. Katz-Downie, C.M. Valiejo-Roman, E.I. Terentieva, A.V. Troitsky, B.Y. Lee, J. Lahham and A. Ei-Oqlah. “Tribes and clades within Apiaceae subfamily Apioideae: the contribution of molecular data.” Edinburgh Journal of Botany 58(2001): 301-330.Print

Downie, S.R., D.S. Katz-Downie, F.J. Sun and C.S. Lee. “Phylogeny and biogeography of Apiaceae tribe Oenantheae inferred from nuclear rDNA ITS and cpDNA psbI–5’trnK(UUU) sequences, with emphasis on the North American Endemics clade.” Botany 86(2008): 1039–1064. Print

Downie, S.R., K. Spalik, D.S. Katz-Downie and J. Reduron. “Major clades within Apiaceae subfamily Apioideae as inferred by phylogenetic analysis of nrDNA ITS sequence.” Plant Diversity and Evolution 128.1-2 (2010): 111-136. Print

Drude, C.G.O. Umbelliferae. In: Engler, A. and Prantle, K. (eds), Die Naturlichen Pflanzenfamilian, Vol. 38. W. Engelmann, Germany, 1897-1898, pp. 63-250.

Jimenez-Mejias, P. and P. Vargas. “Taxonomy of the tribe Apieae (Apiaceae) revisited as revealed by molecular phylogenies and morphological characters.” Phytotaxa 212.1 (2015): 57-79. Print

Kondo, K.., S. Terabayashi, M. Okada, C. Yuan and S. He. “Phylogenetic relationship of medicinally important Cnidium officinale and Japanese Apiaceae based on rbcL sequence.” Journal of Plant Research 109 (1996): 21-27. Print

Magee, A.R., C.I. Calvino, M. Liu, S.R. Downie, P.M. Tilney and B.E. Van Wye. “New tribal deliminations for the early diverging lineages of Apiaceae subfamily Apioidae.” Taxon 59(2010): 567– 580. Print

Pimenov, M.G. and M.V. Leonoy. The genera of the Umbelliferae: A Nomenclature. Royal Botanic Garden, Kew, UK, 1993. Print

Plunkett, G.M., D.E. Soltis and P.S. Soltis. “Higher level relationship of Apiales (Apiaceae and Araliaceae) based on rbcL sequence.” American Journal of Botany 83(1996a): 499-515. Print

Plunkett, G.M., D.E. Soltis and P.S. Soltis. “Evolutionary pattern in Apiaceae: Inferences based on matK sequence data.” Systematic Botany 21(1996b): 477-495. Print

Shan, R.H. and L. Constance. “The genus Sanicula (Umbelliferae) in the old world and the new.” University of California Publications in Botany 1951, 25: 1-78. Print

Spalik, K., S.R. Downie and M.F. Watson. “Generic delimitations within the Sium alliance (Apiaceae tribe Oenantheae) inferred from cpDNA rps16–5’trnK(UUU) and nrDNA ITS sequences.” Taxon 58(2009): 735–748. Print

Sun, F.J. and S.R. Downie. “Phylogenetic relationships among the perennial, endemic Apiaceae subfamily Apioideae of western North America: additional data from the cpDNA trnF-trnL-trnT region continue to support a highly polyphyletic Cymopterus.” Plant Diversity and Evolution 128(2010): 151–172.Print

Tilney, P.M., B.E. Van Wyk, S.R. Downie and C.I. Calvino. “Phylogenetic relationships in the genus Lichtensteinia (Apiaceae) based on morphological, anatomical and DNA sequence data.” South African Journal of Botany 75(2009): 64–82. Print

Valiejo-Roman, C.M., E.I. Terentieva, M.G. Pimenov, E.V. Kljuykov and T.H. Samigullin. Broad polyphyly in Pleurospermum s.l. (Umbelliferae-Apioideae) or broad homoplasy in nrDNA ITS and chloroplast sequences?. In: Pimenov, M.G. and Tilney, P.M. (eds), Apiales 2008, The programme and proceedings of the 6th International Symposium on Apiales. KMK Sci Press Ltd, Moscow, 2008, pp. 140–143.

Winter, P.J.D., A.R. Magee, N. Phephu, P.M. Tilney, S.R. Downie and B.E. Van Wyk. “A new generic classification for African peucedanoid species (Apiaceae).” Taxon 57(2008): 347–364.Print

Zhou, J., H. Peng, S.R. Downie, Z.W. Liu and X. Gong. “A molecular phylogeny of Chinese Apiaceae subfamily Apioideae inferred from nuclear ribosomal DNA internal transcribed spacer sequences.” Taxon 57(2008): 402–416.Print




DOI: https://doi.org/10.21746/aps.2017.6.10.3



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