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Induced drought and salinity tolerance in the variants of Brassica campestris (L.).

Gopal Krishna Shukla


The variants obtained from M2 generation of gamma irradiated toria varieties, T-9 and  PT303 of Brassica campestris (L.) were evaluated for drought and salt tolerance. Among them, variant TV4 of var. T-9 and the variants PD3 and PD4 of PT303 showed significantly higher antioxidant enzyme activities, proline content, relative water content percentage and yield per plant under these stress conditions. These variants appear more adaptive which may be assigned to their induced favorable genotypic alterations, and could be utilized for developing drought and salinity tolerant Brassicas.


Brassica campestris (L.), drought, salinity, stress, tolerance, variants.

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Asharaf, M., and T. McNeilly. “Responses of four Brassica spp. to sodium chloride.” Environmental and Experimental Botany 30. (1990): 475-487.Print.

Aspinal, D., and L.D. Pelag. “In the physiology and biochemistry of drought resistance in plants” Eds. Pelag, L.G., and D. Aspinal. Academic press. Sydeny (1981): 215-228.Print.

Alia, P., Pardhasaradhi and P. Mohonty. “Proline in relation to free redicals production in seedlings of B. juncea raised under sodium chloride stress.” Plant and soil 155 (1993): 497-500.Print.

Barr, L.S., and P. Weatherly. “A reexamination of the relative turgidity techniques for estimating water deficit in leaves”. Australian J. of BioSciences 15(1962): 413-428.Print.

Bates, L.S., R.P. Waldern and I.D. Teere. “Rapid determination of free proline for water stress studies.” Plant and soil 39(1973): 205-207.Print.

Euller, H.Von, Josephson et al. “Uberkatalase I leibeg.” Annals of chemistry 452 (1927): 158-187.Print.

Gosset, D.R., E.P.Millholon and M.C. Lucas. “Antioxidant response to NaCl stress in salt tolerant and salt sensitive cultivars of cotton.” Crop Science 34 (1994):706-714.Print.

Hewitt, E.J. “Sand and water culture method used in study of plant nutrition.” Commonwealth Agricultural Bureaux. Farnham Royal Bank. U.K. (1968): 105-110.Print.

Koca, H. et al., “The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content in Seasame indicum.” Environment and Experimental Botany 60 (2007): 344-351.Print.

Kraus, E., B.D. McKersie and R.A. Fletcher. “Achobutrazol induced tolerance of wheat leaves to paraquot may involve increased antioxidant enzyme activity.” Journal of plant physiology 145 (1995): 570-576.Print.

Lowry, O.H., N.J. Rosenberough, A.L. Farr and Randall. “Protein measurement with the folin-phenol reagent.” Journal of Biological chemistry 193 (1951): 265-275.Print.

Luck, H., H.U. Bergmeyer. “Peroxidase In methods in enzymetic analysis.” Acadmic press Inc, Newyork. (1974): 895-897.Print.

Madan, S., H.S. Nainawatiee et al., “Leaf position dependent changes in proline-5 carboxylase reductase activity and water relation under salt stressing genetically stable salt tolerant somaclones of Brassica juncea L.” Plant Soil 163 (1994): 151-156.Print.

Mishra, N., A. Gupta. “Effect of salt stress on proline metabolism in two high yielding genotypes of green gram.” Plant Science 169 (2005): 331-339.Print.

Mittova, V., M. Guy et al., “Salinity upregulates the antioxidant system in root mitochondria and peroxisome in the wild salt tolerant tomato species Lycopersion pennilli.” Journal of Experimental Botany 55 (2004): 1105-1113.Print.

Munns, R. “Comparative physiology of salt and water stress.” Plant cell and Environment. 25 (2002): 239-250.Print.

Sairam, R.K., and G. Srivastva. “Changes in antioxidant activity in sub cellular fraction of tolerant and susceptible wheat genotypes in response to long term water stress.” Plant Science 162 (2002): 897-904.Print.

Sairam, A.K., and A. Tyagi. “Physiology and molecular biology of salinity tolerance in plants.” Current science 86 (2004): 407-421.Print.

Shrivalli, B., G. Sharma and R. Khanna Chopra. “Antioxidant defense system in upland rice culture subjected to water stress followed by recovery.” Physiologia Plantarum 199 (2003): 503-510.Print.

Singh, K.P., and K. Singh. “Influence of stimulated water stress in proline accumulation in Triticum aestivum L.” Indian journal of Plant Physiology 26.3 (1983): 319-321.Print.

Saleik, R., P.G. Yoeng and D.D. Lefobvre. “Mutants of Arabidopsis thaliana capable of germination under saline conditions.” Plant Physiology 101 (1993): 839-845.Print.

Zhang, J. and M.N.B. Kirkham. “Antioxidant response to drought in sunflower and sorghum seedling.” Phytologia 132 (1964): 361-362.Print.

Zhou, J.K.. “Plant salt tolerance.” Trends plant science 6 (2001): 66-71.Print.


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