Study of total phenol, flavonoid contents and phytochemical screening of methanolic crude extracts of two weed plants

Shah Shreshtha, Jain Anushi, Ambika Nitesh Joshi*, Nitesh Joshi, Harshal Anupma


Weeds have been neglected and their use for medicinal purpose has not been considered on a large scale. Our current research was based on the phytochemical Screening of Ipomoea carnea and Alternanthera sessilis and to quantitatively evaluate their total phenolic and flavonoid content. Quantitative analysis of Phenols and Flavonoids was done by the Folin Ciocalteau and Aluminium chloride method respectively. Both weeds showed positive response for presence of secondary metabolites like flavonoids, phenols, tannins, terpenoids, proteins, carbohydrates, etc. which indicates that they can be used as herbal components. The total Phenol value of Ipomoea carnea and Alternanthera sessilis was 0.0526 and 0.0657 mg Gallic acid equivalent/g respectively. The flavonoid content value of Ipomoea carnea and Alternanthera sessilis was 0.09 and 0.2 mg quercetin equivalent (QE) /g respectively. Both the weeds showed presence of several phytochemicals and high Phenol and Flavonoid values. However, Alternanthera showed a higher concentration of Phenols and Flavonoids as compared to Ipomoea. Since both the selected weeds grow in many areas throughout the world, their medicinal properties can be exploited and used for several applications. With the continuous use of antibiotics, microorganisms have become resistant. There is a need to develop alternative antimicrobial drugs using weeds, which otherwise have not been exploited till now by humans.


Alternanthera; Flavonoid Content; Ipomoea; Phenol Content; Phytochemicals

Full Text:



Doss, Preliminary phytochemical screening of some Indian Medicinal Plants, Ancient Science of Life, 2009, 29(2),12 -16.

Abhinav A. Mali and M. B. Kanade, Allelopathic effect of two common weeds on seed germination, root-shoot length, biomass and protein content of jowar, Annals of Biological Research, 2014, 5 (3):89-92.

AhlemRebaya, Souad Igueld Belghith, Béatrice Baghdikian, Valérie Mahiou Leddet, Fathi Mabrouki, Evelyne Olivier, Jamila kalthoum Cherif, Malika Trabelsi Ayadi, Total Phenolic, Total Flavonoid, Tannin Content, and Antioxidant Capacity of Halimiumhalimifolium (Cistaceae), Journal of Applied Pharmaceutical Science, 2014, 5 (01), 052-057.

D.E. Okvu, Phytochemicals, Vitamins and Mineral Contents of Two Nigerian Medicinal Plants, International Journal of Molecular Medicine and Advance Sciences, 2005, 1(4), 375-381

Esha rami, Illapatel, Quantitative analysis of total phenols and flavonoids in In vivo and In vitro samples of oroxylumindicum(l.) vent, Asian J Pharm Clin Res, 2014, 8(2),202-204.

Jain, A., Roy, S., Joshi, A., & Joshi, N., Evaluation of In-vitro cytotoxic and antioxidant activity of methanolic extracts of Ipomoea carnea and Alternanthera sessilis. International Journal of Bioassays, 2016, 5(08), 4763-4766.

K. Sahayaraj, C. Ravi, Preliminary Phytochemistry of Ipomea carnea jacq. andvitexnegundolinn. leaves, Int. J. Chem. Sci.,2008, 6(1), 1-6.

Khatiwora, E., B. Adsul, V., M. Kulkarni, M., R. Deshpande, N., & V Kashalkar, R., Spectroscopic determination of total phenol and flavonoid contents of Ipomoea carnea. International Journal of Chemtech Research, 2010, 2(3), 1698-1701.

LalithaSree T and K. Vijayalakshmi, Phytochemical screening and quantitative analysis of bioactive components in various extracts of Alternanthera sessilis linn Leaves, IJCPS, 2016, 4(5): 242–246.

ManjulikaYadav, SanjuktaChatterji, Sharad Kumar Gupta and GeetaWatal, Preliminary Phytochemical screening of six medicinal plants used in TraditionaL Medicine, International Journal of Pharmacy and Pharmaceutical Sciences, 2014, 6(5).

Mohamed Abd El-AalLotfySadek, Impact of the Invasive Ipomoea carnea Jacq. on Plant Diversity Along the Canal and Drain Banks of Nile Delta, Egypt, CATRINA,2014, 11 (1), 33-40.

Mohammed, Salmin K. Alshalmani and AwadGiummaAbdellatif, Antioxidant and Quantitative Estimation of Phenolic and Flavonoids of Three Halophytic Plants Growing in Libya Hamdoon, Journal of Pharmacognosy and Phytochemistry, 2013, 2 (3): 89-94.

N. Savithramma, M. Linga Rao and D. Suhrulatha, Screening of Medicinal Plants for Secondary Metabolites, Middle-East Journal of Scientific Research, 2011, 8 (3): 579-584.

Nitesh Joshi, Neha Nangia and Ambika Joshi, Seed germination studies on allelopathic effects of weeds on vignaradiata L, Int. J. Bioassays, 2015, 4 (02), 3664-3666

Patil, M., and SaheraNasreen. "Screening for Secondary Metabolites of Some Important Medicinal Plants". Paripex - Indian journal of research, 2015, 4.8.

R. Sivakumar and D. Sunmathi, Phytochemical Screening and Antimicrobial activity of ethanolic leaf extract of Alternanthera sessilis (L.) and Alternanthera philoxeroides (mart.) 2016, 3(3), 409-412.

S. Komathi and M. FernandusDurai, Qualitative Phytochemical Screening of SaccharumspontaneumLinn. Leaf Extracts, Journal of Academia and Industrial Research (JAIR), 2015, 4(6),168-170

Sharma, A and Bachheti, RK., A review on Ipomoea carnea. Int J Pharm BioSci, 2013, 4(4), 363 – 377.

Singh, A., Kandasamy, T and Odhav, B., In vitro propagation of Alternanthera sessilis (sessile joyweed), a famine food plant. African Journal of Biotechnology, 2009, 8 (21), 5691-5695.

Stanley Irobekhian Reuben Okoduwa, Ismaila A Umar, Dorcas B James, Hajara M Inuwa, and James D Habila, Evaluation of extraction protocols for anti-diabetic phytochemical substances from medicinal plants, World J Diabetes, 2016,7(20), 605–614.

Tekeshwar Kumar, Vishal Jain, Appraisal of Total Phenol, Flavonoid Contents, andAntioxidant Potential of Folkloric Lanneacoromandelica Using In vitro and In vivo Assays, Hindawi Publishing CorporationScientifica, 2015, Volume 2015, 1-3.

UdayaPrakash NK, Bhuvaneswari S, Aravind R, Kaviyarasan V, Kalaivanan K, Hariram SB, A Comparative study on antibacterial activity of common weeds, IJPBS, 2011, 2(1), 677-683.

V. Nandagoapalan, A. Doss and C. Marimuthu, Phytochemical Analysis of Some Traditional Medicinal Plants, Bioscience Discovery, 2016, 7(1):17-20.


Copyright (c) 2017 Annals of Plant Sciences

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.