Micropropagation of B. monnieri using humin media in plant tissue culture

Suman Kashyap*, Neera Kapoor, Radha D. Kale

Abstract


Humus is a complex material formed during the breakdown of organic matter. Earthworm castings contain a high percentage of humus. These humic substances are capable of improving plant nutrition and growth, reminiscent of hormones. Residue obtained after acid-base treatment of vermicompost was used as plant tissue culture media for the micropropagation of Bacopa monnieri. Tukey’s Studentized Range (HSD) Test has clearly indicated that development of leaves from nodes was significantly higher in humin alone without any supplements. The F value was 5.5 and Pr > F was 0.0087. The weight of the plantlets (in milligrams) was significantly higher in the humin medium and humin supplemented with vitamins and micronutrients but was least in the humin medium with growth regulators. The difference observed was at the level of < 0. 0001. The explant development on humin alone has shown the significant percentage survival of in vitro micropropagated plantlets when compared with other treatments. Explants responded maximum to humin only but not so on adding the supplements and the level of significance was at Pr > F was < 0.0001. The maximum growth stimulatory effect was found in aqueous extract of vermicompost which can be made out from the Student’s t-test performed to compare the means of weight of plantlets grown on humin and vermicompost extract media with and without chemical supplements respectively. The probability of this result, assuming the null hypothesis is 0.0014. 

Keywords


Humin media; B. monnieri; economical media; micropropagation; vermicompost extract

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References


Cacco G, Dell'Agnola G. Plant growth regulator activity of soluble humic complex. Can J Soil Sci. 1984, 64, 225–228.

Nardi S, Panuccio MR, Abenavoli MR, Muscolo A. Auxin-like effect of humic substances extracted from faeces of Allolobophora caliginosa and A. rosea. Soil Biol Biochem. 1994, 26, 1341–1346.

Masciandaro G, Ceccanti B, Garcia C. Soil agro-ecological management: fert-irrigation and vermicompost treatments. Biores Technol. 1997, 59, 199-206.

Schnitzer M. Soil organic matter: the next 75 years. Soil Sci. 1991, 151, 41–58.

Vaughan D, Malcolm RE. Influence of humic substances on growth and physiological process. In: Vaughan D, Malcolm RE., editors. Soil Organic Matter and Biological Activity. Dordrecht, The Netherlands: Kluwer Academic Publishers. 1985, pp. 37–75.

Chen Y, Aviad T. Effects of humic substances on plant growth. (In: MacCarthy P, Clapp CE, Malcolm RL, Bloom PR., Eds). Humic Substances in Soil and Crop Science: Selected Readings. Madison, WI: American Society of Agronomy Inc., Soil Science of America, Inc. 1990, 161–186.

Atiyeh RM, Lee S, Edwards CA, Arancon NQ, Metzger JD. The influence of humic acids derived from earthworm-processed organic wastes on plant growth. Bioresource Technology. 2002, 84, 7-14.

Canellas L, Olivares F, Okorokova-Façanha A, Façanha A. Humic Acids Isolated from Earthworm Compost Enhance Root Elongation, Lateral Root Emergence, and Plasma Membrane H+-ATPase Activity in Maize Roots. Plant Physiol. 2002, 130, 1951–1957.

Muscolo A, Felici M, Concheri G, Nardi S. Effect of earthworm humic substances on esterase and peroxidase activity during growth of leaf explants of Nicotiana plumbaginifoli. Biomedical and Life Sciences. 1993, 15, 127-131.

Atiyeh RM, Edwards CA, Subler S, Metzger JD. Pig manure vermicompost as a component of a horticultural bedding plant medium: effects on physicochemical properties and plant growth. Bioresour Technol. 2001, 78, 11-20.

Rastogi S, Pal R, Kulshreshtha DK. 1994. Bacoside A3-a triterpenoidsaponins from Bacopa monnieri. Phytochemistry, 1994, 36, 133-137.

National Medicinal Plants Board (NMPB) and Technology Information Forecasting and Assessment Council (TIFAC), Department of Science and Technology, Government of India. 2007.

Tiwari V, Singh BD, Tewari KN. Shoot regeneration and somatic embryogenesis from different explants of Brahmi (Bacopa monniera (L.) Wettst). Plant Cell Rep. 1998, 17, 538–543.

Tiwari V, Tewari KN, Singh BD. Suitability of liquid cultures or in vitro multiplication of Bacopa monnira (L.) Wettst. Phytomorphology. 2000, 50, 337–342.

Tiwari V, Tewari KN, Singh BD. Comparative studies of cytokinins on in vitro propagation of Bacopa monniera. Plant Cell Tissue Organ Cult. 2001, 66, 9–16.

Tiwari V, Tewari KN, Singh BD. Shoot bud regeneration from different explants of Bacopa monniera (L.) Wettst.by trimethoprim and bavistin. Plant Cell Rep. 2006, 25, 629–635.

Shrivastava N, Rajani M. Multiple shoot regeneration and tissue culture studies on Bacopa monnieri (L.) Pennell. Plant Cell Rep. 1999, 18, 919–923.

Tejavathi DH, Shailaja KS. Regeneration of plants from the cultures of Bacopa monnieri (L.) Pennell. Phytomorphology. 1999, 49, 447–452.

Mohapatra HP, Rath SP. In vitro studies of Bacopa monnieri—an important medicinal plant with reference to its biochemical variations. Indian J Exp Biol. 2005, 43, 373-376.

Ramesh M, Saravanakumar RM, Pandian KS. Benzyl amino purine and adenine sulphate induced multiple shoot and root induction from nodal explants of Brahmi, Bacopa monnieri (Linn.) Penn. Nat Prod Radiance. 2006, 5, 44–51.

Praveen N, Naik PM, Manohar SH, Nayeem A, Murthy HN. In vitro regeneration of brahmi shoots using semisolid and liquid cultures and quantitative analysis of bacoside A. Acta Physiol plant. 2009, 31, 723–728.

Ceasar AS, Maxwell LS, Prasad BK, Karthigan M, Ignacimuthu S. Highly efficient shoot regeneration of Bacopa monnieri (L.) using a two-stage culture procedure and assessment of genetic integrity of micropropagated plants by RAPD. Acta Physiologiae Plantarum. 2009, 32, 443-452.

Murashige T, Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiology and plants. 1962, 15, 472-497.

Mathur S, Kumar S. Phytohormone self-sufficiency for regeneration in the leaf and stem explants of Bacopa monnieri. Journal of Medicinal and Aromatic Plant Sciences. 1998, 20, 1056-1059.

Tiwari V, Singh BD, Tewari KN. Shoot regeneration and somatic embryogenesis from different explants of Brahmi (Bacopa monniera (L.) Wettst). Plant Cell Rep. 1998, 17, 538–543.

Tiwari V, Tewari KN, Singh BD. Suitability of liquid cultures or in vitro multiplication of Bacopa monnira (L.) Wettst. Phytomorphology. 2000, 50, 337–342.

Banerjee M, Shrivastava S. An improved protocol for in vitro multiplication of Bacopa monnieri (L.). World J Microbiol Biotechnol. 2008, 8, 1355–1359.

Shrivastava N, Rajani M. Multiple shoot regeneration and tissue culture studies on Bacopa monnieri (L.) Pennell. Plant Cell Rep. 1999, 18, 919–923.

Muscolo A, Felici M, Concheri G, Nardi S. Effect of earthworm humic substances on esterase and peroxidase activity during growth of leaf explants of Nicotiana plumbaginifoli. Biomedical and Life Sciences. 1993, 15, 127-131.

Kashyap S, Kapoor N, Kale RD. Callus Induction and Tissue Differentiation of Tinospora cordifolia on Using Vermicompost and its Extracts along with Coelomic Fluid as Tissue Culture Media Horizon Journal of Micro. Biotech. Res. 2015 1(1): 001-007. http://www.horizonjournals.org/hjmbr/content/2015/august/august-content.php.




DOI: https://doi.org/10.21746/aps.2017.05.004

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