Antimicrobial and larvicidal potential of endophytic fungi isolated from Tragia involucrata Linn.

Ananda Danagoudar, Chandrashekhar G. Joshi*, M. T. Nivya, H. M. Manjunath, Jagadeesha Poyya, R. Sunil Kumar

Abstract


Endophytic fungi are one of the untapped resources of therapeutic compounds for various diseases. The present study focused on the antimicrobial as well as larvicidal activity of ethyl acetate extract of endophytic fungi isolated from Tragia involucrata. The ethyl acetate extract of Penicillium citrinum CGJ-C1 (GenBankNo.KT780618), Penicillium citrinumCGJ-C2 (KP739821), Cladosporiumsp. (KP739822), and Cryptendoxyla hypophloia CGJ-D2 (KT780619) was subjected to antimicrobial activity against a panel of microorganisms by disc diffusion method, larvicidal activity against Culex quinquefasciatus. All the extracts showed significant antimicrobial activity against the tested organisms ranging from 8±0.32 to 13±2.11mm (zone of inhibition). The extent of activity was comparable to the standard drugs. The larvicidal potential of the endophytes was superior to T. involucrata extract. The larvicidal activity was found to be dose and time dependent with LC50 value ranging from 4.25- 158.06 ppm after 24hrs of treatment. This is the first report on the bioactivity of the endophytes isolated from T. involucrata. Further studies on the bio-guided isolation of lead compound will benefit the people suffering from microbial diseases.


Keywords


Tragia involucrata, Endophytic Fungi, Antimicrobial, Antiyeast, Larva.

Full Text:

PDF

References


Arora DS, Kaur J. Antimicrobial activity of spices. Int. J. Antimicrob. Agents. 12, (1999): 257–262. doi:10.1016/S0924-8579(99)00074-6.

Bhattacharya K, Chandra G. Phagodeterrence, larvicidal and oviposition deterrence activity of Tragia involucrata L.(Euphorbiaceae) root extractives against vector of lymphatic filariasis Culex quinquefasciatus (Diptera: Culicidae). Asian Pac. J. Trop. Dis. 4, (2014): S226–S232.

Cui J, Guo S, Xiao P. Antitumor and antimicrobial activities of endophytic fungi from medicinal parts of Aquilaria sinensis. J. Zhejiang Univ. Sci. B 12, (2011): 385–392.

Darsie RF, Samanidou-Voyadjoglou A. Keys for the identification of the mosquitoes of Greece. J. Am. Mosq. Control Assoc. 13, (1997): 247–254.

Farook SM, Atlee WC. Antidiabetic and hypolipidemic potential of Tragia involucrata Linn. In streptozotocin-nicotinamide induced type II diabetic rats. Int. J. Pharm. Pharm. Sci. 3, (2011): 103–109.

Genus Culex - Florida Medical Entomology Laboratory [WWW Document], n.d. URL http://fmel.ifas.ufl.edu/key/genus/culex_quin.shtml (accessed 8.20.16).

Higginbotham SJ, Arnold AE, Ibañez A, Spadafora C, Coley PD, Kursar TA. Bioactivity of fungal endophytes as a function of endophyte taxonomy and the taxonomy and distribution of their host plants. PloS One 8, (2013): e73192.

Joshi CG, Gopal M, Byregowda SM. Cytotoxic activity of Tragia involucrata Linn. extracts. Am.-Eurasian J. Toxicol. Sci. 3, (2011a): 67–69.

Joshi CG, Gopal M, Kumari NS. Antitumor activity of hexane and ethyl acetate extracts of Tragia involucrata. Int. J. Cancer Res. 7, (2011b): 267–277.

Joshi GC, Gopal M. Antifertility Activity of Hexane and Ethyl Acetate Extracts of Aerial Parts of Tragia involucrata. Linn. J. Pharmacol. Toxicol. 6, (2011): 548–553. doi:10.3923/jpt.2011.548.553

Katoch M, Singh G, Sharma S, Gupta N, Sangwan PL, Saxena AK. Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell (Scrophulariaceae). BMC Complement. Altern. Med. 14, (2014): 52. doi:10.1186/1472-6882-14-52

Liang H, Xing Y, Chen J, Zhang D, Guo S, Wang C. Antimicrobial activities of endophytic fungi isolated from Ophiopogon japonicus (Liliaceae). BMC Complement. Altern. Med. 12, (2012): 1.

Matasyoh JC, Dittrich B, Schueffler A, Laatsch H. Larvicidal activity of metabolites from the endophytic Podospora sp. against the malaria vector Anopheles gambiae. Parasitol. Res. 108, (2011): 561–566. doi:10.1007/s00436-010-2098-1

Rajasekaran A, Duraikannan G. Larvicidal activity of plant extracts on Aedes Aegypti L. Asian Pac. J. Trop. Biomed. 2, (2012): S1578–S1582. doi:10.1016/S2221-1691(12)60456-0

Strobel G, Daisy B. Bioprospecting for Microbial Endophytes and Their Natural Products. Microbiol. Mol. Biol. Rev. 67, (2003): 491–502. doi:10.1128/MMBR.67.4.491-502.2003

Supaphon P, Phongpaichit S, Rukachaisirikul V, Sakayaroj J. Antimicrobial Potential of Endophytic Fungi Derived from Three Seagrass Species: Cymodocea serrulata, Halophila ovalis and Thalassia hemprichii. PLOS ONE 8, (2013): e72520. doi:10.1371/journal.pone.0072520

Tamokou J de D, Chouna JR, Fischer-Fodor E, Chereches G, Barbos O, Damian G, Benedec D, Duma M, Efouet APN, Wabo HK, Kuiate JR, Mot A, Silaghi-Dumitrescu R. Anticancer and Antimicrobial Activities of Some Antioxidant-Rich Cameroonian Medicinal Plants. PLoS ONE 8. (2013): doi:10.1371/journal.pone.0055880

Teke GN, Lunga PK, Wabo HK, Kuiate J-R, Vilarem G, Giacinti G, Kikuchi H, Oshima Y. Antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of Entada abyssinica Stend ex A. Satabie. BMC Complement. Altern. Med. 11, (2011): 57. doi:10.1186/1472-6882-11-57

Zhao J, Zhou L, Wang J, Shan T, Zhong L, Liu X, Gao X. Endophytic fungi for producing bioactive compounds originally from their host plants. Curr Res Technol Educ Trop Appl Microbiol Microb. Biotechnol 1, (2010): 567–576.




DOI: https://doi.org/10.21746/aps.2017.01.002



Copyright (c) 2016 Annals of Plant Sciences

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.