In Vitro Shoot Multiplication of Flacourtia jangomas (Lour.) Raeusch

Abstract

Shoot bud multiplication was obtained in Flacourtia jangomas, a dioecious woody plant of medicinal as well as pomocultural value, by using nodal segment and shoot apex explants. The basal medium was MS medium supplemented with 3.0% sucrose and 0.8% agar. The optimum PGRs for establishment of culture were 1.0mg/l Kinetin and 0.1mg/l NAA. Maximum shoot buds were noted when transferred in MS medium with 5.0mg/l BAP and 0.1mg/l NAA for shoot multiplication. The cycle was repeated twice and 78-81 shoot buds was obtained per explants. Rooting was noted after 60 days in half strength MS with 3.0mg/l NAA. It was also obtained within 15 days by transferring shoot lets after a week from higher conc. (3.0mg/l) to a lower conc. (1.0mg/l) of NAA. After transferring to pots, about 40% of them survived.