Ni-inhibited seedling growth in Zea mays (maize) involves alterations in associated biochemical processes

Asha Kumari Negi

Abstract


An experiment was carried out to investigate the effect of Nickel (Ni) (5–50 µM) on seedling growth, dry weight, chlorophyll content and carotenoid content of Zea mays L. (maize; Var. Aruna 5) grown in petries after 96 h exposure to Ni. The experiment was further extended to investigate the effect of Ni on water soluble protein content and the activities of protease and oxidoreductase enzymes (POD, PPO) in the roots of maize exposed to Ni. The results indicated that Ni inhibits both root and coleoptile length in test plant followed by reduction in chlorophyll and carotenoid contents. Decrease in the activity of proteases and protein content was also observed in test plant. The reduction was observed more in Z. mays roots as compared to coleoptile when treated with Ni. Further, significant reduction in chl b was noted more than chl a in the test plant. However, the increased activities of peroxidase (POD) and polyphenol oxidase (PPO) were observed with increase in Ni concentrations in Z. mays roots.


Keywords


Nickel; Toxicity; enzymes; Zea mays; chlorophyll

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References


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DOI: https://doi.org/10.21746/aps.2016.06.001

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