Ni-inhibited seedling growth in Zea mays (maize) involves alterations in associated biochemical processes

Asha Negi

Abstract


An experiment was carried out to investigate the effect of Nickel (Ni) (5–50 µM) on seedling growth, dry weight, chlorophyll content and carotenoid content of Zea mays (maize) grown in petries after 96 h exposure to Ni.  The experiment was further extended to investigate the effect of Ni on water soluble protein content and the activities of protease and oxidoreductase enzymes (POD, PPO) in the roots of maize exposed to Ni. The results indicated that Ni inhibits both root and coleoptile length in test plant followed by reduction in chlorophyll and carotenoid contents. Decrease in the activity of proteases and protein content was also observed in test plant. The reduction was observed more in Z. mays roots as compared to coleoptile when treated with Ni. Further, significant reduction in chl b was noted more than chl a in the test plant. However, the increased activities of peroxidase (POD) and polyphenol oxidase (PPO) were observed with increase in Ni concentrations in Z. mays roots.


Keywords


Nickel; Toxicity; enzymes; Zea mays; chlorophyll

References


Eskew D. L., R. M. Welch, and E. E. Cary. “Nickel: An Essential Micronutrient for Legumes and Possibly All Higher Plants.” Science, 1983, 222, 621–623.

Kabata-Pendias A., and H. Pendias. “Biogeochemistry of Trace Elements.” PWN, Warszawa, Poland, 1999, 398 p (in Polish).

Mishra D., and M. Kar. “Nickel in plant growth and metabolism.” Botanical Review 1974, 40, 395–452.

Krupa Z., A. Siedlecka, W. Maksymiec, and T. Baszynski. “In vivo response of photosynthetic apparatus of Phaseolus vulgaris to nickel toxicity.” Journal of Plant Physiology, 1993, 142,664–668.

Gajewska E., M. Sklodowska, M. Slaba, and J. Mazur. “Effect of nickel on antioxidative enzyme activities, proline and chlorophyll contents in wheat shoots.” Biologia Plantarum, 2006, 50, 653–659.

Lowry O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall “Protein estimation with Folin-phenol reagent.” Journal of Biological Chemistry, 1951, 193, 265–275.

Basha S. M. M., and L. Beevers. “The development of proteolytic activity and protein degradation during the germination of Pisum sativum L.” Planta, 1975, 124, 77–87.

Batish D. R., H. P. Singh, N. Setia, S. Kaur, and R. K. Kohli. “Effect of 2-Benzoxazolinone (BOA) on the seedling growth and associated biochemical changes in mung bean (Phaseolus aureus).” Zeitschrift für Naturforschung, 2006, 61c, 709–714.

Van Lelyveld L. J., and W. Pretorious. “Assay methods of determining enzymatic activity of -amylase, indole-3-acetic acid, polyphenol oxidase, peroxidase and ascorbic acid oxidase in a crude extract from avocado tree bark.” Agrochemophysica, 1973, 51, 29–34.

Hiscox J. D., and G. F. Israelstem. “A method for the extraction of chlorophyll from leaf tissue without maceration.” Canadian Journal of Botany, 1979, 57, 1332–1334.

Arnon, D. I. "Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris." Plant Physiology, 1949, 24, 1-15.

Rani, D. and R. K. Kohli. "Fresh matter is not an appropriate relation unit for chlorophyll content: Experience from experiments on effects of herbicide and allelopathic substance." Photosynthetica, 1991, 25, 655-658.

Espen L., L. Pirovano, and S. M. Cocucci. “Effects of Ni2+ during the early phases of radish (Raphanus sathnts) seed germination.” Environment and Experimental Botany, 1997, 38, 187–197.

Jagetiya B. L., and N. I. Aery. “Effect of different nickel salts on germination in mung.” Environmental Development, 1998, 189–192.

Kowalczyk J., J. Borkowska-Burnecka, and K. Cieslak. “Heavy metals accumulation in greenhouse tomatoes.” Acta Horticulturae, 2003, 613, 57–60.

Singh S., R. Saxena, K. Pandey, K. Bhatt, and S. Sinha. “Response of antioxidants in sunflower (Helianthus annuus L.) grown on different amendments of tannery sludge: Its metal accumulation potential.” Chemosphere, 2004, 57, 1663–1673.

Ahmad M. S. A., M. Hussain, M. Ashraf, R. Ahmad, and M. Y. Ashraf. “Effect of nickel on seed germinability of some elite sunflower (Helianthus annuus L.) cultivars.” Pakistan Journal of Botany, 2009, 41, 1871–1882.

Baccouch S., A. Chaoui, and E. EL Ferjani. “Nickel-induced oxidative damage and antioxidant responses in Zea mays shoots.” Plant Physiology and Biochemistry, 1998, 36, 689–694.

Baccouch S., A. Chaoui, and E. El Ferjani. “Nickel toxicity induces oxidative damage in Zea mays roots.” Plant Nutrition, 2001, 24, 1085–1097.

Gajewska E., M. Sklodowska, M. Slaba, and J. Mazur. “Effect of nickel on antioxidative enzyme activities, proline and chlorophyll contents in wheat shoots.” Biologia Plantarum, 2006, 50, 653–659.

Maheshwari R., and R. Dubey. “Inhibition of ribonuclease and protease activities in germinating rice seeds exposed to nickel.” Acta Physiologiae Plantarum, 2008, 30, 863–872.

Kumar H., D. Sharma, and V. Kumar. “Nickel induced oxidative stress and antioxidant defense in barley roots and leaves.” Environmental Biology, 2012, 2, 121–128.

Liu D., J. Zou, Q. Meng, J. Zou, and W. Jiang. “Uptake and accumulation and oxidative stress in garlic (Allium sativum L.) under lead phytotoxicity.” Ecotoxicology, 2009, 18, 134–143.

Malecka A., A. Piechalak, I. Morkunas, and B. Tomaszewska. “Accumulation of lead in roots cells of Pisum sativum.” Acta Physiologiae Plantarum, 2008, 30, 629–637.

Demchenko N. P., I. B. Kalimova, and K. N. Demchenko. “Effect of nickel on growth, proliferation and differentiation of root cells in Triticum aestivum seedlings.” Russian Journal of Plant Physiology, 2005, 52, 220–228.

Diaz J., A. Bernal, F. Pomar, and F. Merino. (2001) “Induction of shikimate dehydrogenase and peroxidase in pepper (Capsicum annum L) seedlings in response to copper stress and its relation to lignification.” Plant Science, 2001, 161, 179–188.

Dubey D., and A. Pandey. “Effect of nickel (Ni) on chlorophyll content, lipid peroxidation and antioxidant enzymes activities in black gram (Vigna mungo) leaves.” International journal of science and nature, 2011, 2, 395–401.

Awasthi K., and P. Sinha. “Nickel stress induced antioxidant defence system in sponge guard (Luffa cylindrical).” Journal of Plant Physiology and Pathology, (2013) 1, doi: 10.4172/1000102.

Latif H. H. “The Influence of Nickel Sulphate on Some Physiological Aspects of Two Cultivars of Raphanus sativus L.” Archives of Biological Science Belgrade, 2010, 62, 683–691.

Rai V., P. Vajpayee, S. N. Singh, and S. Mehrotra. “Effect of chromium accumulation on photosynthetic pigments, oxidative stress defense system, nitrate reduction, proline level and eugenol content of Ocimum tenuiflorum L.” Plant Science, 2004, 167, 1159–1169.

Khatun S., M. B. Ali, E. J. Hann, and K. Y. Paek. “Copper toxicity in Withania somnifera: Growth and antioxidant enzymes reaponses of in vitro grown plants.” Environmental and Experimental Botany, 2008, 64, 279–285.

Prasad D. D. K., and A. R. K. Prasad. “Effect of lead and mercury on chlorophyll synthesis in mung bean seedlings.” Phytochemistry, 1987, 26, 881.

Kevresan S., N. Petrovic, M. Popovic, and J. Kandrac. “Effect of heavy metals on nitrate and protein metabolism in sugar beet.” Biologia Plantarum, 1998, 41, 235–240.

Demirevska-Kepova K., L. Simova-Stoilova, Z. Stoyanova, R. Ho¨lzer, and U. Feller. “Biochemical changes in barley plants after excessive supply of copper and manganese.” Environmental and Experimental Botany, 2003, 52, 253–266.

Shah K., and R. S. Dubey. “Effect of Cd on proline accumulation and ribonuclease activity in rice seedlings- role of proline as a possible enzyme protectant.” Plant Biology, 1997, 40, 121–130.

Balestrasse K. B., M. P. Benavides, S. M. Gallego, and M. L. Tomaro. “Effect of Cd stress on nitrogen metabolism in nodules and roots of soybean plants.” Functonal plant biology, 2003, 30, 57–64.

Simonovicova M., L. Tamas, J. Huttova, and I. Mistrik. “Effect of aluminum on oxidative stress related enzymes activities in barley roots.” Biologia Plantarum, 2004, 48, 261–266.

Alam M. M., S. Hayat, B. Ali, and A. Ahmad. “Effect of 28- homobrassinolide treatment on nickel toxicity in Brassica juncea.” Photosynthetica, 2007, 45, 139–142.

Batish D. R., H. P. Singh, S. Kaur, R. K. Kohli, and S. S. Yadav. “Caffeic acid affects early growth, and morphogenetic response of hypocotyl cuttings of mung bean (Phaseolus aureus).” Plant Physiology, 2008, 165, 297–305.




DOI: https://doi.org/10.21746/aps.2016.06.001

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Annals of Plant Sciences

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.