Impact of phopshate solubilizing bacteria (Bacillus polymixa) on drought tolerance of green gram [Vigna radiata (L.) Wilczek]

Vidya P., Shintu V. P.*, Jayaram M. K.

Abstract


The main focus of the present study is to evaluate the effect of priming of green gram (Vigna radiata) with phosphate solubilising bacteria (PSB) during drought stress. Drought is the major abiotic stress factor which diminishing the growth and development of agricultural in Kerala. So immediate steps need to be taken to overcome the adverse effect of drought stress for the development of agriculture. Phosphate Solubilizing Bacteria are one of the best microorganisms found to be simultaneously increasing the insoluble soil Phosphorus uptake by the plant and crop yield. In the study, the seeds of Vigna radiata were subjected to priming treatment with 0.5 % and 1% phosphate solubilising bacteria. Physiological and biochemical parameters like germination percentage, root and shoot length, relative water content (RWC), amount of chlorophyll, protein, proline and yield were studied.  Inoculation with phosphate solubilising bacteria showed remarkable variation in both physiological and biochemical parameters of green gram plants. Among the two concentrations tested, 1% phosphate solubilising bacteria was found to be effective in mitigating the effect of water stress, stimulating early flowering and also in increasing yield.

Keywords


Germination; Relative Water Content; Chlorophyll; Protein; Proline.

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References


Neumann, P.M. “Coping mechanism for crop plants in drought-prone environments”. Annalsof Botany 101, 7(2008): 901-907.

Shao, H.B., L.Y. Chu, C.A. Jaleel and C.X. Zhao. “Water deficit stress – induced anatomical changes in higher plants”. Comptes Rendus Biologies 331, 3 (2008): 215-225.

Kramer, P.J. “Plant and Soil water relationships: A modern synthesis”. Mc Graw Hill Book Co., New York, 1969, p 482.

Ceccarelli, S. “Plant responses to water stress: A review”. Genetica Agraria 38 (1984): 43-74.

Agarwal, R.M., P. Rashmi and G. Sunitha. “Certain aspects of water stress induced changes and tolerance mechanisms in plants”. Journal of Indian Botanical Society 78, 3-4(1999): 255-269.

Xiong, L. and J.K. Zhu. “Molecular and genetic aspects of plant responses to osmotic stress”. Plant, Cell and Environment 25, 2 (2002): 131-139.

Shafi, M., J. Bakht, M.J. Hassan, M. Raziuddin and G. Zhang. “Effect of cadmium and salinity stresses on growth and antioxidant enzyme activities of wheat (Triticum aestivum L.)”. Bulletin of Environmental Contamination and Toxicology 82, 6(2009): 772-776.

Chivasa, W., D. Harris, C. Chiduza, A. B. Mashingaidze and P. Nyamudeza. “Determination of optimum on farm seed priming time for maize (Zea mays L.) and sorghum (Sorghum bicolor) for use to improve stand establishment in semi arid agriculture”. Tanzanian Journal of Agricultural Science 3,2 (2015): 103–112.

Harris, D., A. Rashid, S. Ali and P.A. Hollington ‘On farm’ seed priming with maize in Pakistan. Proceedings of 8th Asian regional maize workshop: New technologies for the new millennium held at Bangkok, Thailand, Cimmyt, Mexico,(2004) pp. 316–321.

Shintu, P.V. and K.M. Jayaram. “Phosphate solubilising bacteria (Bacillus polymyxa)-An effective approach to mitigate drought in tomato (Lycopersicon esculentum Mill.).” Tropical plant research 2 (2015): 17-22.

Ravikumar S., S. Shanthy, A. Kalaiarasi and S. Sumaya. “Effect of halophytic phosphobacteria on Avicenia officinalis seedlings”. Annals of Biological Research 1, 4 (2010): 254-260.

Gaur, A.C. “Phosphate solubilizing micro organisms as biofertilizers”, New Delhi: Omega Scientific Publishers, 1990, p.176.

Chhonkar, P.K. “Biological strategies for nutrient cycle in soil system” In: B.D Kaushik (Ed.) Soil plant microbe interaction in Relation to integrated Nutrient Management, IARI, New Delhi, 1998, pp 48-54.

Lagreid M., O.C. Bockman, O. Kaarstad. Agricultural Fertilizers and the environment. CABI Publishing. Oxon, UK, 1999, p.294.

Bars H.D. and P.E.Weatherly. “A re-examination of relative turgidity technique for estimating water deficits in leaves”. Australian Journal of Biological. Sciences 15, 3 (1962):413-428.

Arnon D.T. “Copper enzymes in isolated chloroplasts poly phenol oxidase in Beta vulgaris”. Plant Physiology 24, 1 (1949): 1-15.

Lowry O.H., R. J. Rosenbrough, A.L. Farr and R. J. Randall. “Protein measurement with Folin phenol reagent”. Journal of Biological Chemistry 193, 1 (1951): 265-275.

Bates L., R. P. Waldren and I.D. Teare. “Rapid determination of free proline for water stress studies”. Plant Soil 39, 1(1973): 205-207.

Sharma K., G. Dak, A. Agrawal, M. Bhatnagar and R. Sharma. “Effect of phosphate solubilizing bacteria on the germination of Cicer arietinum seeds and seedling growth”. Journal of Herbal Medicine and Toxicology 1, 1 (2007): 61-63.

Kadkhodaie, A., M. Bagheri. “Seed treatment during germination in Linseed to overcome salt and drought stresses (Linum usitatissimum L.)”. World Academy of Science, Engineering and Technology 73 (2011): 373-377.

Basaglia, M., S. Casella, V. Peruch, S. Poggiolini, T. Vamerali, G. Mosca and P. Vanderlayden. “Field release of genetically marked Azospirillum brasilense in association with Sorghum bicolor L.” Plant and Soil 2003, 256, 2 (2003): 71-75.

Dobbeleaere, S., A. Croonenborghs, A. Zhus, D. Ptacck and J. Vanderleyden. “Responses of Agronomically important crops to incoulation with Azospirillum”. Australian Journal of Plant Physiology 28, 9 (2001): 871-879.

Bahadhur, V. and T. Singh. “Yield and growth responses of garden pea (Pisum sativum) to nitrogen and phosphorus application”. Vegetable Science 17, 2 (1990): 205-209.

Nanjundappa, G., B. Shivaraj, S. Sridhar, S. Janarjuna. “Effect of organic and inorganic sources of nutrients alone and in combination on the growth and yield of fodder maize”. Mysore Journal of Argicultural Sciences 34, 3 (2000): 247-250.

Yordanov, I., V.Velikova and T. Tsonev. “Plant response to drought and stress tolerance”. Bulgarian Journal of Plant Physiology (Special Issue), (2003): 187-206.

Valentovic, P., M.Luxora, L. Kolarovic and O. Gasparikova. “Effect of osmotic stress on compatible solutes content, membrane stability and water relations in two maize cultivars”. Plant Soil and Environment 52, 4 (2006): 186-191.

Singh, D. and N. B. Singh. “Water stress tolerance in fenugreek (Trigonella foenum-graecum L.) Inoculated with Bacillus polymyxa, a phosphate solubilizing bacterium”. Journal of Indian Botanical Society 89, 1 (2010): 86-91.

Jiang, Y. and B. Huang. “Protein alterations in tall fescue in response to drought stress and abscic acid”. Crop Science 42, 1 (2002): 202-207.

Duan, H.G., S.Yuan, W. J. Liu, D.H. Xi. D. H. Qing and H.G. Liang. “Effects of Exogenous spermidine on photo system II of wheat seedlings under water stress”. Journal of Integrative Plant Biology 48, 8 (2006): 920-927.

Zgallai, H., K. Steppe and R. Lemeur. “Photosynthetic, physiological and bio chemical responses of tomato plants to polyethylene glycol- induced water deficit”. Journal of Integrative Plant Biology 47, 12 (2006): 1470-1478.

Ambreen, A., A. Hisamuddin and S. Rushada. “Antagonistic effects of Pseudomonas fluorescens and Bacillus subtilis on Meloidogyne incognita infecting Vigna mungo L.”. International Journal of Plant, Animal, Environmental Sciences 2 (2012): 223-229.

Riccardi, F., P. Gazeau, D. Vienne. “Protein changes in response to progressive water deficit in maize, quantitative variation and polypeptide identification”. Plant Physiology 117, 4 (1998): 1253-1263.

Radin, J.W. “Stomatal response to water stress and to abscisic acid in phosphorus deficient cotton plants”. Plant Physiology 76, 2 (1984): 392-394.

Hanson, A.P. and W.P. Hitz. “Metabolic response of mesophytes to plant water deficit”. Annual Review of Plant Physiology 33, 1 (1982): 163-203.

Sankar, B., A.J. Cheruth, M. Paramasivam, K. Ashok, S. Ramamurthi and P. Rajaram. “Drought induced biochemical modifications and proline metabolism in bhendi (Abelmoschus esculentus L.)”. Acta Botanica Croatica 66, 1 (2007): 43-56.

Saravanakumar, D., M. Kavino, T. Raguchander, P. Subbian and R. Samiyappan. “Plant growth promoting bacteria enhance water stress resistance in green gram plants”. Acta Physiologiae Plantarum 33, 1 (2011): 203-209.

Casanovas, E.M., C.A. Barassi and R.J. Sueldo. “Azospirillum inoculation of maize seed during inhibition”. Cereal Research Communication 28 (2003): 25-32




DOI: https://doi.org/10.21746/aps.2016.04.001

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