Phytochemical investigation on various parts of Psidium guajava

Abstract

The objective of the study was to perform phytochemical screening of alcoholic and aqueous extracts of leaves, seeds and fruit pulp of Psidium guajava, to isolate the active principles by column chromatography and to characterize the isolated compound by chemical tests and IR spectroscopy. The phytochemical investigation was performed to identify the presence of glycosides, flavanoids, alkaloids, saponins, vitamin, tannins, carbohydrate, amino acid, steroid in the extracts, which showed phytoconstituents in various proportions in aqueous and alcoholic extracts of seeds, fruit pulp and leaves of Psidium guajava. Leaf extract showed the presence of maximum number of phytoconstituents and alcoholic fruit pulp extract showed minimum constituents.  Alcoholic seed extracts were subjected to column chromatography and qualitative chemical test and IR spectroscopy of the isolated compound showed that the compound may be a steroidal glycoside or the compound has a steroidal nucleus with a lactone ring.