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Antifungal activity of essential oil and leaf extract of Adenocalymma alliaceum and its role in management of stem end rot disease of mango fruits caused by fungal pathogen Botryodiplodia theobromae

Pramila Tripathi

Abstract


Study was carried out for evaluation of antifungal activity of essential oil and extract of plants for control of stem end rot of mango. To investigate antifungal activity leaves of 20 angiospermic taxa were extracted separately in water, acetone, ethyl acetate, ethyl alcohol, methanol and petroleum ether and screened against Botryodiplodia theobromae that cause stem end rot of mango fruits. Plant Adenocalymma alliaceum leaf extract in water, acetone, ethyl alcohol, petroleum ether, ethyl acetate and methanol was found to exhibit strongest antifungal activity. The essential oil of A. alliaceum was also found fungi toxic against other fruit rotting fungi. The volatile fraction of the leaves of A. alliaceum was isolated in the form of essential oil and was standardized through physicochemical and fungitoxic properties. The minimum inhibitory concentration (MIC) of the oil was found to be 100ppm. The oil was thermostable in nature and had a long shelf life of 2 years. The oil was found to withstand high inoculum density. An in vivo trial on mango fruits (Dashehari) was conducted with aqueous extract and essential oil of A. alliaceum. Aqueous extract enhanced the shelf life of mango fruits up to 5 days. Fumigation of the fruits with the oil of A. alliaceum enhanced the shelf life up to 8 days by protecting them from fungal rotting.

Keywords


Antifungal; Garlic creeper; essential oil; fungicidal; postharvest diseases; stem end rot

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References


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