Cover Image

Application of molecular markers to improve drought tolerance and yield enhancement in upland rice

Saumya Awasthi*, J. P. Lal

Abstract


Upto now, a lot of drought related genes were cloned and individual gene showed positive effects under controlled stress experiments, but were not effective in the field. However, the progresses by conventional breeding approaches were achievable as some drought varieties have been released to the farmers in the recent years. Although, this is not adequate to cope up with the future demand of high yield for rice, as drought seems to spread to more regions and seasons across the country. Therefore, marker assisted selection came into lime light for accelerating and giving pace to plant breeding. The identification of QTLs with a major effect on grain yield raises a new hope of improving grain yield under drought through marker assisted breeding. The availability of the major effect QTL for drought tolerance, a theoretical frame-work for marker assisted selection and the existence of intolerant varieties that are widely accepted by farmers provides an opportunity to develop cultivars that would be suitable for larger areas of drought prone rice. In case of (Sarjoo - 52 × Birsa Gora) × Sarjoo - 52, two drought tolerant genes were incorporated. Only those plants were therefore selected which showed polymorphism for the genes MQTL1.1 and qDTY3.2. Thus, plant number SA – D – 1, SA – D – 7, SA- D – 8, SA – D – 9, SA – D – 13, SA – D - 27 and SA – D – 56 were selected. These lines have been subjected to further breeding and trial tests. Agronomic performances and physiological behavior of these lines are also under track. The results showed that the variety Sarjoo – 52 could be efficiently converted to a drought tolerant variety in a backcross generation followed by selfing and selection, involving a time of two to three years. Polymorphic markers for foreground and background selection were identified for the high yielding variety to develop a wider range of drought tolerant variety to meet the needs of farmers in the drought-prone regions. This approach demonstrates the effective use of marker assisted selection for a major QTL in a molecular breeding program.

Keywords


Oryza sativa; drought; marker assisted selection; QTLs; foreground selection; background selection.

Full Text:

PDF

References


Babu RC, Nguyen BD, Chamarerk V, Shanmugasundaram P, Chezhian P, Jeyaprakash P, Ganesh SK, Palchamy A, Sadasivam S, Sarkarung S, Wade LJ and Nguyen HT, Genetic analysis of drought resistance in Rice by molecular markers, Association between Secondary Traits and Field Performance, Crop sci, 43, 1457- 1469, 2003.

Bernardo R, 2002, Breeding for quantitative traits in plants, Stemma Press, Woodburn, Minnesota,

Blum A, Plant Breeding for Stress Environments, CRC Press, Boca Raton, FL, 1988.

Boyer JS.1982. Plant productivity and environment. Science. 218: 443.

Chen S, Lin XH, Xu CG, Zhang Q. Improvement of bacterial blight resistance of “Minghui 63’, an elite restorer line of hybrid rice, by molecular marker assisted selection. Crop Sci. 40(1): 239- 244. 2000.

Dekkers JCM, commercial application of marker and gene assisted selection in livestock: stategies and lessons, Paper presented at the 54th annual meeting of the European association for animal production, Rome, Italy, 31st August – 3rd September.

Edmeades GO, Bolanos J, Lafitte HR, Rajaram S, Pfeiffer W and Fischer RA. Traditional approaches to breeding for drought resistance in cereals, In, Baker, F,W,G, (ed,), Drought Resistance in Cereals, CAB International, Wallingford, Oxon, UK, 27-52, 1989.

Francia E, Tacconi G, CrosattiC, Barabaschi D, Bulgarelli D, Dall’Aglio E and Vale G, Marker assisted selection in crop plants. Plant Cell Tissue Organ Cult, 82, 317- 342, 2005.

Frisch M, Bohn M and Melchinger AE. Minimal sample size and optimal positioning of flanking markers in markr assisted backcrossing for transfer of a target gene. Crop sci. 39: 967- 975.1999a.

Frisch M and Melchinger AE. Selection theory for Marker assisted backcrossing. Genet. 170: 909- 917. 2005.

Fukai S, Pantuwan G, Jongdee B and Cooper M, Screening for drought resistance in rainfed lowland rice, Field Crop Res, 64, 61-74, 1999.

Jongdee B, Fukai S and Cooper M, Leaf water potential and osmotic adjustment as physiological traits to improve drought tolerance in rice, Field Crop Res, 76, 153-163, 2002.


Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Annals of Plant Sciences

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.