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Meristem culture for rapid regeneration in Black pepper (Piper nigrum Linn.)

Umadevi P.*, K. V. Saji, E. J. Suraby


The shoot apical meristem for production of plantlets from black pepper variety Sreekara was assessed. Meristem extension was achieved in liquid medium containing Murashige and Skoog with 0.1 mgl-1 kinetin and 0.5 mgl-1 GA3, subsequent direct shoot induction in ½ MS with 3 mgl-1 BA and 1 mgl-1 IAA followed by shoot growth and development in ½ MS +0.5 mgl-1Indole butyric acid. The meristems of 2mm resulted in shoot induction and proliferation via direct organogenesis. Successful rooting of meristem derived shoots was achieved in half strength WPM with 3 mgl-1 BA and 1 mgl-1 Kinetin. In vitro multiplication from the meristem derived plants shows the possibility for mass multiplication. The developed protocol was effective with 40% regeneration frequency of meristems, simple and rapid protocol for regeneration with 66-70days and subsequent micro propagation for the production of plant stock. This is the very first report on meristem culture in blackpepper. The protocol will be of immense importance in rapid mass multiplication of elite germplasm, as well as for conservation of this important species.


Blackpepper; Meristem culture; Direct organogenesis.

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Babu KN, Rema J, Zachariah TJ, Samsudeen CK & Ravindran PN, Plant regeneration from tissue cultures of Piper colubrinum L., J Plant Crops, 24 (1996) 594-596.

Bhat AI, Devasahayam S, Venugopal MN & Suseela Bhai R, Distribution and incidence of viral disease of black pepper (Piper nigrum L.) in Karnataka and Kerala, India, J Plant Crops, 33 (2005) 59-64.

Bhat SR, Chandel KPS & Malik SK, Plant regeneration from various expalnts of cultivated Piper species, Plant Cell Rep 14 (1995) 398-402.

de Silva DPP, Jones P & Shaw MW, Identification and transmission of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper(Piper nigrum L.) in Sri Lanka, Plant Pathol, 51 (2002) 537-545.

Hareesh PS & Bhat AI, Detection and partial nucleotide analysis of Piper yellow mottle virus infecting black pepper in India, Indian J virol, 19 (2008) 160-167.

Helliot B, Panis B, Paumay Y, Swenen R, Lepoivre P & Frison E, Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (musa spp.), Plant cell Rep, 20 (2002) 1117-1122.

Kane ME, Shoot culture procedure. In: Trigiano RN, Gray DJ (eds) Plant development and biotechnology. (CRC Press LLC, Boca Raton) 2005, 145-157.

Lloyd G & Mccown BH, Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot tip culture. Int.Plant Prop. Soc., Comb. Proc., 30: 421-427.

Murashige T& Skoog F, A revised medium for rapid growth and bioassay using tobacco tissue cultures, Physiol Plant, 15 (1962) 473-497.

Nazeem PA, Augustin M, Rathy K, Sreekumar PK & Rekha CR, A viable protocol for large scale in vitro multiplication of black pepper. (P. nigrum L.), J Plant Crops, 32 (2004) 163-168.

Philip VJ, Dominic J, Triggs GS & Dickinson NM, Micro propagation of black pepper (Piper nigrum Linn.) through shoot tip cultures, Plant cell Rep, 12 (1992) 41-44.

Ravindran PN Botany and crop improvement. In: Ravindran PN (ed) Black pepper: Piper nigrum L. (Hardwood Academic publishers, The Netherlands), 2000, 1-526.

Sharma YR & Kalloo G, Status of current research towards increased production and productivity in black pepper in India, Focus on pepper, 1 (2004) 69-86.

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