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Induction of sporocarp development in vitro in the mosquito fern, Azolla rubra R. Br.

Sini S., Smitha R. B., P. V. Madhusoodanan*

Abstract


Azolla rubra R. Br. (Azollaceae: Pteridophyta), a popular biofertilizer applied in inundated paddy fields propagate usually through vegetative fragmentation only. Induction of sporocarp development, callus, and indirect organogenesis were accomplished. Influence of various factors such as agar, light, phosaphate, sucrose and pH on in vitro growth of A. rubra were also studied. Half strength MS medium with 2.26 µM 2,4-Dichlorophenoxyacetic acid and 6.6 µM 6-Benzyl Amino Purine was found best for callus induction. The callus transferred onto medium having 6.6 µM BA underwent indirect organogenesis. Shoot tips cultured in light on medium having 2.26 µM 2,4-Dichlorophenoxyacetic acid and 6.6 µM 6-Benzyl Amino Purine with 0.8% agar and 3 % sucrose at pH 5.8 was found best for induction of callus.

Keywords


Azolla rubra R. Br.; sporocarp induction; in vitro development

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