Use of Polymerase Chain Reaction for the Detection of Banana Bunchy Top Virus Infection in Three Successive Generation of Banana

Jai Godheja*, Sudhir K. Shekhar, D. R. Modi, Chatterjee T.


Banana is one of the most important fruit in India. Viral infection causes a great loss in Banana production with a ratio of about 20-30% loss and occasionally reaching 50-80%. The most indigenous banana are infected by BBTV; Banana bunchy top virus, a complex circular single-stranded DNA virus with multiple genomic components. On mature plants infected with BBTV, they appear to be “bunched” at the top of the plant, new leaves emerge with difficulty, are narrower than normal, the symptom for which this disease is named. For the early detection of BBTV, PCR technique was used for the plant samples. Suckers were used for isolating genomic DNA using sodium sulphite method. DNA was quantified (400 ng/µl) and then PCR was used for detecting BBTV by using BBTV specific primers. The agarose gel was loaded with ladder DNA, followed by sample DNA. Among 3 DNA samples all showed amplification by specific primers of BBTV. During the course of my practical it has been clear that the third generation plant of banana was also infected by BBTV. Therefore it is very important to detect the presence of these virus early in infected plant and proper eradication of infectious plant, before it passes to another healthy plants by its vector Pentagonia nigronervosa.


Banana; BBTV; sucker

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