Induction of In-Vitro Flowering and Callogenesis in Blepharis maderaspatensis L. (Acanthaceae), A Medicinal Plant

Drisyadas P., Smitha R. B.*, Sailas Benjamin, P. V. Madhusoodanan


Explants from node, internode and leaf of Blepharis maderaspatensis L. were cultured on MS medium supplemented with various growth regulators for in vitro callogenesis, organogenesis and flowering. MS medium with 4.53µM 2, 4-D was most effective for callus induction from leaf explant. Flowering was induced in vitro when axillary shoots developed on MS medium (with 2.85 µM IAA) were subcultured on MS medium fortified with 8.06 µM NAA and 5.71 µM IAA. 75% of plants survived in field conditions.


Blepharis maderaspatensis; Callogenesis; in vitro rooting; propagation

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Udayan PS, Balachandran I, Medicinal plants of Arya VaidyaSala Herb Garden. Arya Vaidya Sala Kottakkal, Kerala, 2011.

Neelambika HS, Leelavathi S, In vitro comparative study of membrane stabilization capacity of different extracts of Blepharis maderaspatensis (L.) Heyne Ex. Roth. and Blepharis mollunginifolia Pers. grown in the region of Mysore, Karnataka, International Journal of Pharmaceutical Sciences and Research, 2014, 5, 2698-2702.

Ayyanar M, Ignacimuthu S, Herbal medicines for wound healing among tribal people in southern India: Ethnobotanical and Scientific evidences, International Journal of Applied Research in Natural Products, 2009, 3, 29-42.

Shanmugam S, Gayathri SN, Sakthivel B, Ramar S, Rajendran K, Plants used as medicine by Paliyar Tribes of Shenbagathope in Virudhunagar District of Tamilnadu, India, Ethnobotanical Leaflets, 2009, 13, 370-78.

Subitha KT, Ayyanar M, Udayakumar M, Sekar T, Ethnomedicinal plants used by Kani tribals in Pechiparai forests of Southern western Ghats, Tamil Nadu, India, International Research Journal of Plant Science, 2011, 12, 349-354.

Mathur A, Joshi H, Ethnobotanical studies of the Tarai Region of Kumaun, Uttarakhand, India, Journal of Plants, People and Applied Research, 2013, 11, 175 – 203.

Sundaresan S, Senthilkumar B, A survey of traditional medicinal plants from the Vellore District, Tamil Nadu, India, International Journal of Ayurvedic and Herbal Medicine, 2013, 5, 1347–1355.

Parotta JA, Healing plants of Peninsular India, CABI publishing, UK, 2001, 26-27.

Murashige T, Skoog F, A revised medium for rapid growth and bioassays for tobacco tissue cultures, Plant Physiology, 1962, 15, 473-497.

Shu QY, Liu GS, Qi DM, Chu CC, Liu J, Li HJ, An effective method for axillay bud culture and RAPD analyis of cloned plants in tetraploid black locust, Plant Cell Reports, 2003, 22, 175-180.

Tyagi RK, Yusuf A, Dua P, Agarwal A, In vitro plant regeration from genotype conservation of eight wild species of Curcuma. Biologia Plantarum, 2004, 48, 129-132.

Faisal M, Anis M, Rapid in vitro propagation of Rauwolfia tetraphylla an endangered medicinal plant. Physiology and Molecular Biology of Plants, 2002, 8, 295-299.

McCartan SA, Staden JV, Micropropagation of endangered Kniphora leucocephala Baijinath. In vitro Cellular and Developmental Biology-Plant, 2003, 5, 496-499.

Nair GI, Seeni S, In vitro multiplication of Calophyllum apetalum (Clusiaceae), an endemic medicinal tree of Western Ghats. Plant Cell Tissue and Organ Culture, 2003, 2, 169-174.

Hu CY, Wang PJ, Meristem shoot tip and bud cultures In: Evans DA, Sharp WR, Ammirato PV, Yamada Y, Hand Book of Plant Cell Culture Vol. 1. Techniques of propagation breeding, (Macmillian Publ. Co. New York) 1983, 177-277.

Beena MR, Martin KP, Kirti B, Hariharan M, Rapid in vitro propagation of medicinally important Ceropegia candelelabrum, Plant Cell Tissue and Organ Culture, 2003, 72, 285-288.

Khan S, Naz S, Ali K, Zaidi S, Direct organogenesis of Kalanchoe tomentosa (Crassulaceae) from shoot-tips. Pakistan Journal of Botany, 2006, 38, 977-981.

Mahesh A, Thangadurai D, Melchias G, Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. Ex Kuntze. Biological Research, 2012, 45, 131-136.

Rani A, Prasad MP, Studies on the organogenesis of Atropa belladonna in in-vitro conditions. International Journal of Biotechnology and Bioengineering Research, 2013, 4, 457-464.

Askari-Khorasgani K, Mortazaeinezhad F, Otroshy M, Reza A, Golparver, Moeini A, Direct regeneration of an endangered medicinal plant Kelussia odoratissima, International Journal of Agriculture and Crop Sciences, 2013, 5, 1969-1974.

Ghimire BK, Seong ES, Kim EH, Lamsal K, Yu CY, Chung IM, Direct shoot organogenesis from petiole and leaf discs of Withania somnifera (L.), African Journal of Biotechnology, 2010, 9, 7453-7461.

Babaei N, Abdullah NAP, Saleh G and Abdullah TL (2014). An Efficient In Vitro Plantlet Regeneration from Shoot Tip Cultures of Curculigo latifolia, a Medicinal Plant. The Scientific World Journal, 2014, Article ID 275028, 9 pages, doi:10.1155/2014/275028.

de Klerk GJ, Brugge JT, Marinova S, Effectiveness of IAA, IBA and NAA during adventitious root formation in vitro in Malus Jorkg. Plant Cell Tissue and Organ Culture, 1997, 49, 39-44.

Dias MS, Almeida R, Romano A, Rapid clonal multiplication of Lavendula viridis through in vitro axillary shoots proliferation, Plant Cell Tissue and Organ Culture, 2002, 68, 99-102.

Biond S, Medium composition and methyl jasmonate influence the amount and spectrum of secondary metabolites in callus cultures of Zanthoxylum stenophyllum Hemsl, Plant Biosystems, 2004, 2, 117-124.

Zhang Q, Jiyanjun C, Richard JH, Regeneration of Syngonium podophyllum variegatum through direct somatic embryogenesis. Plant Cell Tissue and Organ Culture, 2004, 84, 181-188.

Haque M, Ghosh B, Micropropagation, in vitro flowering and cytological studies of Bacopa chamaedryoides, an ethno-medicinal plant, Environmental and Experimental Biology, 2013, 11, 59–68.

Nagarjana A, Arivalaganb U, Rajagurua P, In vitro root induction and studies on antibacterial activity of root extract of Costus igneus on clinically important human pathogens. Journal of Microbiology and Biotechnology Research, 2011, 1, 67-76.

Mohanta YK, Sahoo S, In vitro culture of highly valuable medicinal plant Bacopa Monnieri (L.) penn. for rapid and mass multiplication. International Journal of Pharmaceutical Science Invention, 2014, 3, 41-45.

Handro W, Effect of some growth regulators on in vitro flowering of Streptocarpus nobilis, Plant Cell Reports, 1983, 2, 133-136.

Chithra M, Martin KP, Sunandakumari C, Madhusoodanan PV, Silver nitrate induced rooting and flowering in vitro on rare rheophytic woody medicinal plant, Rotula aquatics Lour., Indian Journal of Biotechnology, 2003, 3, 418 – 421.

Sharma V, Kamal B, Srivastava N, Dobriyal AK, Jadon VS, In vitro flower induction from shoots regenerated from cultured axillary buds of endangered medicinal herb Swertia chirayita H. Karst. Biotechnology Research International, 2014, Article ID 264690, 5 pages, doi:10.1155/2014/264690.

Aileni LM, Kokkirala VR, Yarra R, Vemunoori A, Kasula K, Umate P, Abbagani S, In vitro regeneration, flowering and seed formation from leaf explants of Scoparia dulcis. Medicinal and Aromatic Plant Science and Biotechnology, 2011, 5, 11-14.

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