Expression Studies of Selected Gene Candidates and Isolation of Total Leaf Proteins of Rhizophora mucronata Lam. in Response to Salinity

Abstract

Expression studies help to know the activity of genes and proteins in response to a set of conditions at a given time. In the study, the expression of selected genes, BADH (Betaine Aldehyde Dehydrogenase), NHX1, P5CS (Pyrroline-5-carboxylate synthase), Cytochrome P450, Abscisic acid (ABA) encoding gene, Nicotinamide Adenine Dinucleotide Phosphate Hydrogen (NADPH), Salt Overly Sensitive (SOS1) and Aquaporin were analyzed in Rhizophora mucronata Lam., their expression analysis under different salt concentration was done by using real-time PCR technique and the genes showed early and delayed expression. The gene products were sequenced and the partial sequence was obtained. Total leaf proteins in Rhizophora mucronata Lam. at different saline conditions were isolated and analyzed using two - dimensional gel electrophoresis.