Antimicrobial Studies of some Ganoderma Species against some Gram-Negative Bacterial Isolates of Diabetic Foot Ulcer

Abstract

Despite the diversity within the Ganodermataceae family for its therapeutic applications, this study primarily focuses on five test species of Ganoderma i.e. G. applanatum, G. boninense, G. lucidum, G. resinaceum and G. tsugae. These species are known to have various biologically active macromolecules including polysaccharides, triterpenoids, steroids, phenolic compounds, lipids and alkaloids which are isolated from the fruiting bodies, mycelia and spores. These molecules have various health benefits, including anti-tumoral, anti-inflammatory, anti-allergic, anti-viral, anti-bacterial, antidiabetic and antioxidant properties. In the present study, inhibitory and antibacterial properties of several Ganoderma extracts in petroleum ether, chloroform, acetone, ethanol, methanol and aqueous against, were tested against Gram negative bacterial isolates of DFU. like Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhimurium and Proteus vulgaris by agar well diffusion method. For every examined bacterium, every extract showed a different level of inhibition. Comparative study reveals that G. lucidum and G. tsugae had the highest performance against Gram negative test bacteria revealing its application for creating innovative drug formulations for people with diabetes.