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Development and characterization of novel set of polymorphic SSR markers for Dill (Anethum graveolens L.)

Geetika Jethra, Sharda Choudhary, Vinay Sharma

Abstract


Dill (Anethum graveolens L.), belonging to the Apiaceae family, is considered one of the most important and economically important seed spice grown and cultivated all over India. But, due to minimal genetic and genomic resources, most of the past studies were based on physiological and biochemical analysis rather than molecular breeding. In the present study an attempt was made to develop and design in-silico and computationally derived highly informative microsatellite markers. Current investigation was the first attempt to develop and design microsatellite markers from Dill EST and genomic sequences. A total of 1556 SSRs were found with a density of 1SSR/0.38 kb in 589.390 kb sequence searched. Of the total, 1265, 81.30% were perfect and 291, 18.70% were identified as compound SSRs in nature. Mononucleotides (1089, 69.98%) were most frequent followed by trinucleotides (254, 16.32%), dinucleotides (183, 11.76%), tetranucleotides (22, 1.41%), hexanucleotides (6, 0.38%) and the least frequent were pentanucleotides (2, 0.13%).


Keywords


Anethum graveolens L., Apiaceae, Microsatellite, Simple sequence repeats, Seed Spices

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DOI: https://doi.org/10.21746/aps.2018.7.6.8

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