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Angiotensin-I converting enzyme (ACE-I) inhibitory and antiproliferative potential of chickpea seed protein hydrolysate

Neha Gupta, Sameer Suresh Bhagyawant


Chickpea seeds are the preferred source of proteins possessing health care functions in countries across the world. In the present investigation, the chickpea seed protein subjected to enzymatic hydrolysis produced bioactive peptides that were able to inhibit the angiotensin converting enzyme (ACE) and cytotoxic effect. Experimental screening was to test the efficacy of hydrolytic enzymes in chickpea seed for production of bioactive peptides was therefore carried out. The optimum hydrolysis times for each protein hydrolysate prepared by alcalase which inhibited ACE-I with IC50 value of 52.22 µg/ml. The protein hydrolysate was further subjected to antiproliferative evaluation for breast cancer cell lines MCF-7 and MDA-MB-231 and the IC50 was observed 0.71 mg/ml and 0.78 mg/ml respectively. Study indicated the potential of chickpea proteins as a source of ACE-inhibitory and antiproliferative potential to foresee the application of chickpea proteins into functional foods.


ACE-I inhibitory activity, Chickpea seeds, Hydrolysis

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