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In-silico identification of cis- acting regulatory elements in 5’ regulatory region of Betaine aldehyde dehydrogenase isoenzymes of selected monocot and dicot

Hemani Sharma, Sumit Govil, Divya Shrivastava


It’s challenging to understand the multifarious gene regulatory networks. Diverse biological processes, are mostly conferred by nature of cis-acting regulatory elements (CARE) located within the regulatory region which include abiotic stress responses, hormone responses and developmental processes. Thus, the identification of CARE and their organization is a crucial step for better understanding of spatiotemporal expression of the gene. The putative cis-acting element that are located within the 5' regulatory region of BADH isozymes of Hordeum vulgare , Oryza sativa, Sorghum bicolor, Leymus chinensis, Zoysia tenuifolia, Arabidopsis thaliana, Amaranthus hypochondriacus, Chrysanthemum lavandulifolium, Populus euphratica and Atriplex prostrate were identified using bioinformatics tools. Several probable cis-acting regulatory elements that are coupled with cellular development, hormonal and stress response were identified as: Me-JA, GARE, GT-1, MYB, W-box, I-box, Pyrimidine-box, ANAERO, ARF, CARE, ASF1, SORLIP, SURE, TAAAGSTKST1, GCC Box, CICADIAN, Sp1 and CGCG Box. These results shed light on probable cis-acting regulatory elements these BADH isoenzyme that confer their expression and regulation during cellular, hormonal and stress response.


Biotic and abiotic stress, Betaine aldehyde dehydrogenase (BADH), Cis-acting regulatory elements, Cellular development, Hormonal regulation, Spatiotemporal

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