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Plant Regeneration and Genetic Transformation in Buckwheat (Fagopyrum spp.), A Multipurpose Gluten Free Crop of High Nutraceutical Importance: A Critical Review

Mithilesh Kumar

Abstract


Buckwheat (Fagopyrum sp.) from family Polygonaceae, with its two main cultivated species (F. esculentumMoench. and F. tataricum) is mostly grown in hilly regions of Eurasia. It is a multipurpose gluten free crop having great nutraceutical value. For plant propagation several reports have been published and significant differences were noticed in culture protocols, genotypes and their success rates. A few studies report genetic transformation in buckwheat with varying degree of success. The present study reviews the in-vitro regeneration and genetic transformation reports in Fagopyrum spp. available in publically available literature and concludes on what needs to be done for the sustainable genetic improvement of buckwheat. A genotype independent high frequency in-vitro regeneration protocol compatible with high end stable genetic transformation is the need of the hour for sustained genetic improvement of this crop.


Keywords


Agrobacterium rhizogenes, A. tumefaciens, Buckwheat, Fagopyrum dibotrys, F. esculentum, F. tataricum, Genetic transformation, Hairy root culture, In-vitro regeneration, Organogenesis, Rutin, Somatic embryogenesis.

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References


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DOI: https://doi.org/10.21746/aps.2018.7.1.7

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