Impact of some phytohormones, metabolic inhibitor and agrochemicals on senescing paddy leaves and its relevance to productivity

  • Haseena Rafath Department of Botany, Govt. Arts College, Mutlur, Chidambaram, Tamil Nadu

Abstract

The influence of hormones viz. Indole acetic acid (IAA), Gibberellic acid (GA3), Cytokinins viz. Benzyladenine (BA), Benzimidazole (BZI), biocides viz. 2,4-Dichlorophenoxy acetic acid (2,4-D), Diathane M-45, Dimecron and metabolic inhibitors viz. Cycloheximide (CHI) and Actinomycin-D (Act-D) on senescence in detached paddy (Oryza sativa L.) leaves was studied under dark and illuminated conditions. Cytokinins were found to be more effective senescence retardants in light than in dark while auxins were effective in light. Biocides proved to be milder senescence retardants. Effective concentration, a concentration away from the ineffective and toxic range, chosen from IAA, GA3, BA, BZI, 2,4-D, Diathane M-45 and Dimecron were 25,100, 5, 100, 25, 1500 and 0.50 ppm respectively. Application of Act-D (inhibitor of mRNA synthesis) with in 15 min after detachment and cycloheximide (inhibitor of cytoplasmic protein synthesis) from the start of the incubation resulted in suppression of peroxidase activity both under light and dark. Catalase activity exhibited an inverse relation with peroxidase activity under both the experimental conditions. It is recommended that attempts must be made in a more sophisticated way to study the changes in activities of different enzymes suspected to be linked with leaf senescence at a deeper level using all modern techniques available so that the exact nature of enzymes, the isoenzymes pattern and their relation with yield can be understood. Such studies may also help in future in manipulating the projects for the improvement of this economically important cereal crop.

References

Beevers L, Senescence. In: Plant Biochemistry, 3rd Edition (J. Borner and J.E. Varner, eds.), Acad. Press, New York, 1976, pp. 77-794.

Biddington NL, Thomas TH, Influence of different cytokinins on the transpiration and senescence of excised oat leaves, Physiol. Plant., 1978, 42,369-394.

Birecka H, Chaskes MJ, Goldstein J, Peroxidase and senescence, J. Exp. Bot., 1979, 30,565-573.

Biswas, A.K. and Choudhuri, M.A. 1978. Control of senescence of rice leaf by foliar treatment with essential elements, Sci and Cult., 42,236-240.

Chance B, Maehly, Assay of catalases and peroxidases. In: Methods in Enzymology, Vol. II (S.P. Colowick and N.O. Kaplan, eds.) Acad. Press New York, 1955, pp. 764-775.

Chen YM, Certain aspects of light and plant hormones in control of senescence, Taiwamia, 1972, 17, 81-91.

De Leo P, Sacher JA, Control of ribonuclease and phosphatase by auxin and abscissic acid during senescence of Rheo leaf section, Plant Physiol., 1970,46, 806-811.

Engelbrecht L, Cytokinin in buds and leaves during growth, maturity and ageing (with comparison of two bioassays), Biochem. Physiol. Pflanzen, 1971, 162, 547-558.

Ford TW, Simon EW, Peroxidase and glucose diphosphate dehydrogenase levels in cotyledons of Cucumis sativa L., J Exp. Bot., 1972, 23, 423-431.

Goldthwaite FJ, Laetsch WJ, Regulation of senescence in bean leaf discs by light and chemical growth regulators, Plant Physiol., 1967, 42, 1757-1762.

Gunning BES, Barkley WK, Kinetin-induced directed transport and senescence in detached oat leaves, Nature, 1963, 199, 262-265.

Henry EW, Jordan W, The enzymatic response of pea (Pisum sativum) stem section to applied Indole acetic acid, gibberllic acid and three. Catalase peroxidase and polyphenol oxidase, Z. Pflenzen physiol., 1977, 84, 321-327.

Hsia CP, Kao CH, Soybean leaf disc senescence: Regulation by light. Proc Agric Assn, China, Taipei, Taiwan, New Sevin, 1977, 100, 98-100.

Huff A, Peroxidase catalysed oxidation of chlorophyll by hydrogen peroxide, Phytochem, 1982, 21, 261-265.

Kao CH, Senescence of rice leaves. V. Anti-senescent action of cytokinins, Proc Natl. Sci. Council (Taiwan), 1978, 2, 391398.

Kar M, Feieraband J, Metabolism of activated oxygen in detached wheat and rye leaves and its relevance to the initiation of senescence, Planta, 1984, 160,385-391.

Kar M, Mishra D, Catalase, peroxidase and polyphonol oxidase activities during rice leaf senescence, Plant Physiol., 1976, 57, 315-319.

Kende H, The cytokinins, Intrnatl. Rev. Cytol., 1971, 31, 301-338.

Khan PA, Chitra K, Padhy B. Chemical regulation of senescence in excised leaves of Eleusine coracana, J. Berhampur Univ., 1979, 4(1), 117-120.

Khan PA, Lakshmi N, Padhy B, An estimation of the senescence period of excised rice leaves, Curr Sci., 1978, 13, 19-24.

Kumar KB, Khan PA, Effect of insecticides, oxydemetox-methyl and dimethoate on chlorophyll retention and hydrogen peroxide utilisation in ragi (Eleusine coracana Gaertn. cv PR 202) leaves during senescence, Indian J. Exptl. Biol., 1982a ,20, 889-893.

Kumar BK, Khan PA, Peroxidase and polyphenol oxidase in excised ragi, Eleusine coracana Gaertn. cv PR 2020 leaves during senescence. Indian J Exptl. Biol., 1982b, 20, 412-416.

Kumar KB, Khan Pa, Induction and suppression of RNase activity by growth regulators in senescing ragi leaves, Physiologia Planatarum, 1983, 58, 479-485.

Mishra G, Biswal UC, Factors concerned in leaf senescence. 1. Effects of age, chemicals, petiole and photoperiod on senescence in detached leaves of Hibiscus rosasinensis L., 1973, Bot Gaz, 134,5-11.

Mishra D, and Pradhan PK, Regulation of senescence in detached rice leaves by light, benzimidazole and cyto-kinetins, Exp. Geront., 1973, 8,153-156.

Mishra D, Misra B, Retardation of induced senescence of leaves from crop plants by benzimidazole and cytokinins. Exp. Geront., 1973, 8, 235-239.

Mishra G, Das S, Senescence in isolated leaves of Cestrum nocturnum L., Curr Sci., 1978, 47, 970-971.

Nooden LD, Senescence in the whole plant. In: Senescence in plants (K.V. Thimann, ed.), C.R.C. press, Boca Raton, 1980, pp. 2190258.

Person C, Samborski DJ, Forsyth FR, Effect of bezimidazole on detached wheat leaves, Nature, 1957, 180, 1294-1295.

Parish RW, Studies on senescing tobacco leaf discs with special reference to peroxidase, A. The effects of cutting and of inhibition of nucleic acid and protein synthesis, Planta, 1968, 82, 1-13.

Patro B, Effects of certain chemicals on leaf senescence in rice, M. Phil Thesis, Berhampur University, India, 1984.

Patra MK, Kar M, Mishra D, Catalase activity in leaves and cotyledons during plant development and senescence, Biochem. Physiol. Pflanzen, 1978, 72, 385-390.

Reddy KP, Subhani SM, Khan PA, Kumar KB, Effect of light and benzyladenine on dark treated growing rice leaves (Oryza sativa L.) leaves. II. Changes in chlorophyll content and catalase activity, Physiol. Plant, 1985, 63,79-86.

Richmond AE, Lang A, Effect of kinetin on protein content and survival of detached Xanthium leaves, Science, 1957, 125, 650-651.

Ranjani B, Impact of certain pesticides on excised rice leaves during senescence, M. Phil Thesis, Berhampur Univ., India, 1985.

Samejima TW, McCafe WJ, Tsi Yang T, Reconciliation of alkaline-denatured catalase, Arch. Biochem. Biophys., 1968, 127, 354-360.

Sharma R, Biswal UC, Effect of kinetin and light on peroxidase activity of detached barley leaves, Z. Pflanzen Physiol., 1976, 78, 169-172.

Singh N, Mishra D, The effect of benzimidazole and red light on the senescence of detached leaves of Oryza sativa cv, Ratna Physiol. Plant, 1975, 34, 67.

Skoog F, Armstrong DC, Cytokinin, Ann. Rev. Plant Physiol., 1970, 21, 359-384.

Snell FD, Snell CT, Calorimetric methods of analysis of IV AAA, Yan Nostranl Reinhold Co., New York, 1971, PP. 26-27.

Thimann KV, Telley RM, Krivak BM, The metabolism of oat leaves during senescence. V. Senescence in light, Plant Physiol, 1977, 59, 448-544.

Thimann KV, The senescence of leaves, In: Senescence in plants (K.V. Thimann, ed.), CRC Press, Boca Raton, 1980, pp. 85-116.

Thimann KV, Satler SO, Trippi V, Further extension of leaf senescence, In: Plant growth substances (P.F. Waering ed.) Acad. Press. London, 1982, pp. 350-548.

Thimann KV, The senescence in detached leaves of Trapeolum, Plant Physiol., 1985, 79, 1107-1110.

Thimann KV, Forward. In: Senescence and Ageing in plants (L.D. Nooden and A.C. Leopold, eds.) Acad. Press, New York, 1988, pp. XVII-XXV.

Trippi VS, Leaf and plant ageing by photoperiodic effects in Anagalis and its relation with some enzyme patterns. Phyton, 1980, 38, 23-32.

Verga A, Bruinsma J, Effects of different cytokinins on the senescence of detached oat leaves, Planta, 1973, 111, 91.
Waygood ER, Benzimidazole effect on chloroplast of wheat leaves, Plant Physiol., 1965, 40, 1242-1246.

Woolhouse H, W, Longevity and senescence in plants. Sci Progress (Oxford), 1974, 61, 123-147.

Woolhouse HW, The biochemistry and regulation of senescence in chloroplasts, Canadian J. Bot., 1984, 12, 2934-2942.

Woolhouse HW, Biochemical and molecular aspects of plant senescence, In: Molecular Biology of plant development (H. Smith and D. Grierson, Eds.) Blackwells, Oxford, 1982, PP. 256-287.
Published
2017-05-01
How to Cite
RAFATH, Haseena. Impact of some phytohormones, metabolic inhibitor and agrochemicals on senescing paddy leaves and its relevance to productivity. Annals of Plant Sciences, [S.l.], v. 6, n. 05, p. 1606-1613, may 2017. ISSN 2287-688X. Available at: <http://annalsofplantsciences.com/index.php/aps/article/view/320>. Date accessed: 22 july 2017. doi: https://doi.org/10.21746/aps.2017.05.001.
Section
Research Articles

Keywords

Oryza sativa L. Senescence; auxins; cytokinins; biocides; metabolic inhibitors; catalase; peroxidase